The Solution Structure of the Regulatory Domain of Tyrosine Hydroxylase
MPTPSAPSPQ PKGFRRAVSE QDAKQAEAVT SPRFIGRRQS LIEDARKERE AAAAAAAAAV ASSEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA VKVFETFEAK IHHLETRPAQ RPLAGSPHLE YFVRFEVPSG DLAALLSSVR RVSDDVRSA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 31.2 % (555 of 1777) | 18.9 % (175 of 927) | 36.2 % (252 of 696) | 83.1 % (128 of 154) |
Backbone | 54.7 % (508 of 928) | 44.6 % (139 of 312) | 51.3 % (241 of 470) | 87.7 % (128 of 146) |
Sidechain | 12.5 % (125 of 1001) | 5.9 % (36 of 615) | 23.5 % (89 of 378) | 0.0 % (0 of 8) |
Aromatic | 5.0 % (5 of 100) | 10.0 % (5 of 50) | 0.0 % (0 of 50) | |
Methyl | 21.0 % (37 of 176) | 11.4 % (10 of 88) | 30.7 % (27 of 88) |
1. Ser40 phosphorylated regulatory domain of tyrosine hydroxylase
MPTPSAPSPQ PKGFRRAVSE QDAKQAEAVT SPRFIGRRQS LIEDARKERE AAAAAAAAAV ASSEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA VKVFETFEAK IHHLETRPAQ RPLAGSPHLE YFVRFEVPSG DLAALLSSVR RVSDDVRSASolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7, Details 1 M leupeptin and 1 M pepstatin A were added as protease inhibitor
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Ser40 phosphorylated regulatory domain of tyrosine hydroxylase | [U-95% 13C; U-95% 15N] | 0.8 ~ 1.2 mM | |
2 | D2O | natural abundance | 5 % | |
3 | H2O | natural abundance | 95 % | |
4 | sodium phosphate | natural abundance | 50 mM |