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Solution NMR structure of SLED domain of Scml2
Authors
Bezsonova, I.
Assembly
SLED domain of Scml2
Entity
1. SLED domain of Scml2 (polymer, Thiol state: all free), 119 monomers, 13097.76 Da Detail

GSHMMSTVCV YVNKHGNFGP HLDPKRIQQL PDHFGPGPVN VVLRRIVQAC VDCALETKTV FGYLKPDNRG GEVITASFDG ETHSIQLPPV NSASFALRFL ENFCHSLQCD NLLSSQPFS


Formula weight
13097.76 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.2 %, Completeness: 90.0 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All90.0 % (1227 of 1363)90.5 % (639 of 706)88.4 % (472 of 534)94.3 % (116 of 123)
Backbone95.3 % (663 of 696)95.8 % (228 of 238)94.8 % (330 of 348)95.5 % (105 of 110)
Sidechain86.5 % (672 of 777)87.8 % (411 of 468)84.5 % (250 of 296)84.6 % (11 of 13)
Aromatic33.3 % (40 of 120)35.0 % (21 of 60)31.7 % (19 of 60)
Methyl100.0 % (124 of 124)100.0 % (62 of 62)100.0 % (62 of 62)

1. SLED Scml2

GSHMMSTVCV YVNKHGNFGP HLDPKRIQQL PDHFGPGPVN VVLRRIVQAC VDCALETKTV FGYLKPDNRG GEVITASFDG ETHSIQLPPV NSASFALRFL ENFCHSLQCD NLLSSQPFS

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2


#NameIsotope labelingTypeConcentration
1SLED_Scml2[U-100% 13C; U-100% 15N]0.3 ~ 1.0 mM
2HEPESnatural abundance50 mM
3NaClnatural abundance250 mM
4DTTnatural abundance2 mM
5H2Onatural abundance90 %
6D2O[U-100% 2H]10 %

LACS Plot; CA
Referencing offset: 0.0 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.0 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.17 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.17 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2MEM, Strand ID: A Detail


Release date
2014-04-21
Citation
Solution NMR structure of the DNA-binding domain from Scml2 (sex comb on midleg-like 2)
Bezsonova, I.
J. Biol. Chem. (2014), 289, 15739-15749, PubMed 24727478 , DOI 10.1074/jbc.M113.524009 ,
Related entities 1. SLED domain of Scml2, : 1 : 31 entities Detail
Interaction partners 1. SLED domain of Scml2, : 12 interactors Detail
Experiments performed 10 experiments Detail
NMR combined restraints 4 contents Detail
Keywords PcG, PRC1, Scml2