Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL3)/RsmE(dimer) 2:1 complex
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 59.9 % (645 of 1076) | 64.0 % (365 of 570) | 50.5 % (216 of 428) | 82.1 % (64 of 78) |
Backbone | 50.4 % (310 of 615) | 49.4 % (128 of 259) | 41.8 % (123 of 294) | 95.2 % (59 of 62) |
Sidechain | 75.6 % (393 of 520) | 76.2 % (237 of 311) | 78.2 % (151 of 193) | 31.3 % (5 of 16) |
Aromatic | 9.6 % (10 of 104) | 11.5 % (6 of 52) | 9.5 % (4 of 42) | 0.0 % (0 of 10) |
Methyl | 100.0 % (94 of 94) | 100.0 % (47 of 47) | 100.0 % (47 of 47) |
1. RsmE
MLILTRKVGE SINIGDDITI TILGVSGQQV RIGINAPKDV AVHREEIYQR IQAGLTAPDK RETPH2. SL3(RsmZ)
GGGAUCGCAG GAAGCGAUCC CSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RsmE | [U-100% 15N] | 1 mM | |
2 | SL3(RsmZ) | natural abundance | 1 mM | |
3 | potassium phosphate | natural abundance | 0.05 mM | |
4 | sodium chloride | natural abundance | 0.03 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RsmE | [U-100% 15N] | 1 mM | |
8 | SL3(RsmZ) | natural abundance | 1 mM | |
9 | potassium phosphate | natural abundance | 0.05 mM | |
10 | sodium chloride | natural abundance | 0.03 mM | |
11 | D2O | [U-100% 2H] | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | RsmE | [U-100% 13C; U-100% 15N] | 1 mM | |
13 | SL3(RsmZ) | natural abundance | 1 mM | |
14 | potassium phosphate | natural abundance | 0.05 mM | |
15 | sodium chloride | natural abundance | 0.03 mM | |
16 | H2O | natural abundance | 95 % | |
17 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | RsmE | [U-100% 13C; U-100% 15N] | 1 mM | |
19 | SL3(RsmZ) | natural abundance | 1 mM | |
20 | potassium phosphate | natural abundance | 0.05 mM | |
21 | sodium chloride | natural abundance | 0.03 mM | |
22 | D2O | [U-100% 2H] | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
23 | RsmE | [U-100% 15N] | 1 mM | |
24 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Ade/Ura RNA | 1 mM | |
25 | potassium phosphate | natural abundance | 0.05 mM | |
26 | sodium chloride | natural abundance | 0.03 mM | |
27 | H2O | natural abundance | 95 % | |
28 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
29 | RsmE | [U-100% 15N] | 1 mM | |
30 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Ade/Ura RNA | 1 mM | |
31 | potassium phosphate | natural abundance | 0.05 mM | |
32 | sodium chloride | natural abundance | 0.03 mM | |
33 | D2O | [U-100% 2H] | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
34 | RsmE | [U-100% 15N] | 1 mM | |
35 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Cyt/Gua RNA | 1 mM | |
36 | potassium phosphate | natural abundance | 0.05 mM | |
37 | sodium chloride | natural abundance | 0.03 mM | |
38 | H2O | natural abundance | 95 % | |
39 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
40 | RsmE | [U-100% 15N] | 1 mM | |
41 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Cyt/Gua RNA | 1 mM | |
42 | potassium phosphate | natural abundance | 0.05 mM | |
43 | sodium chloride | natural abundance | 0.03 mM | |
44 | D2O | [U-100% 2H] | 100 % |
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 45.6 % (982 of 2152) | 48.2 % (549 of 1140) | 41.6 % (356 of 856) | 49.4 % (77 of 156) |
Backbone | 43.9 % (540 of 1230) | 48.6 % (252 of 518) | 38.4 % (226 of 588) | 50.0 % (62 of 124) |
Sidechain | 48.2 % (501 of 1040) | 47.7 % (297 of 622) | 49.0 % (189 of 386) | 46.9 % (15 of 32) |
Aromatic | 47.1 % (98 of 208) | 48.1 % (50 of 104) | 45.2 % (38 of 84) | 50.0 % (10 of 20) |
Methyl | 52.1 % (98 of 188) | 52.1 % (49 of 94) | 52.1 % (49 of 94) |
1. RsmE
MLILTRKVGE SINIGDDITI TILGVSGQQV RIGINAPKDV AVHREEIYQR IQAGLTAPDK RETPH2. SL3(RsmZ)
GGGAUCGCAG GAAGCGAUCC CSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RsmE | [U-100% 15N] | 1 mM | |
2 | SL3(RsmZ) | natural abundance | 1 mM | |
3 | potassium phosphate | natural abundance | 0.05 mM | |
4 | sodium chloride | natural abundance | 0.03 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RsmE | [U-100% 15N] | 1 mM | |
8 | SL3(RsmZ) | natural abundance | 1 mM | |
9 | potassium phosphate | natural abundance | 0.05 mM | |
10 | sodium chloride | natural abundance | 0.03 mM | |
11 | D2O | [U-100% 2H] | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | RsmE | [U-100% 13C; U-100% 15N] | 1 mM | |
13 | SL3(RsmZ) | natural abundance | 1 mM | |
14 | potassium phosphate | natural abundance | 0.05 mM | |
15 | sodium chloride | natural abundance | 0.03 mM | |
16 | H2O | natural abundance | 95 % | |
17 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | RsmE | [U-100% 13C; U-100% 15N] | 1 mM | |
19 | SL3(RsmZ) | natural abundance | 1 mM | |
20 | potassium phosphate | natural abundance | 0.05 mM | |
21 | sodium chloride | natural abundance | 0.03 mM | |
22 | D2O | [U-100% 2H] | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
23 | RsmE | [U-100% 15N] | 1 mM | |
24 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Ade/Ura RNA | 1 mM | |
25 | potassium phosphate | natural abundance | 0.05 mM | |
26 | sodium chloride | natural abundance | 0.03 mM | |
27 | H2O | natural abundance | 95 % | |
28 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
29 | RsmE | [U-100% 15N] | 1 mM | |
30 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Ade/Ura RNA | 1 mM | |
31 | potassium phosphate | natural abundance | 0.05 mM | |
32 | sodium chloride | natural abundance | 0.03 mM | |
33 | D2O | [U-100% 2H] | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
34 | RsmE | [U-100% 15N] | 1 mM | |
35 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Cyt/Gua RNA | 1 mM | |
36 | potassium phosphate | natural abundance | 0.05 mM | |
37 | sodium chloride | natural abundance | 0.03 mM | |
38 | H2O | natural abundance | 95 % | |
39 | D2O | [U-100% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
40 | RsmE | [U-100% 15N] | 1 mM | |
41 | SL3(RsmZ) | [U-100% 13C; U-100% 15N]-Cyt/Gua RNA | 1 mM | |
42 | potassium phosphate | natural abundance | 0.05 mM | |
43 | sodium chloride | natural abundance | 0.03 mM | |
44 | D2O | [U-100% 2H] | 100 % |