Solution structure of the circular g-domain analog from the wheat metallothionein Ec-1
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 1:CYS3:SG | 2:CD1:CD |
2 | metal coordination | sing | 1:CYS9:SG | 2:CD1:CD |
3 | metal coordination | sing | 1:CYS13:SG | 2:CD1:CD |
4 | metal coordination | sing | 1:CYS19:SG | 2:CD1:CD |
5 | metal coordination | sing | 1:CYS3:SG | 2:CD1:CD |
6 | metal coordination | sing | 1:CYS7:SG | 2:CD1:CD |
7 | metal coordination | sing | 1:CYS9:SG | 2:CD1:CD |
8 | metal coordination | sing | 1:CYS21:SG | 2:CD1:CD |
9 | metal coordination | sing | 1:GLY31:O | 2:CD1:CD |
10 | peptide | sing | 1:GLY31:C | 1:ALA1:N |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 85.1 % (114 of 134) | 85.1 % (114 of 134) |
Backbone | 80.9 % (55 of 68) | 80.9 % (55 of 68) |
Sidechain | 89.4 % (59 of 66) | 89.4 % (59 of 66) |
Methyl | 88.9 % (8 of 9) | 88.9 % (8 of 9) |
1. cyc-gEc1
AGCDDKCGCA VPCPGGTGCR CTSARSGGAA GSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 307.2 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cyc-gEc1 | natural abundance | 0.9 mM | |
2 | CADMIUM ION | 113Cd | 1.8 mM | |
3 | TRIS | [U-99% 2H] | 10 mM | |
4 | sodium perchlorate | natural abundance | 10 mM |