NMR structure of C-terminal domain from A. ventricosus minor ampullate spidroin (MiSp)
GSGNSVTSGG YGYGTSAAAG AGVAAGSYAG AVNRLSSAEA ASRVSSNIAA IASGGASALP SVISNIYSGV VASGVSSNEA LIQALLELLS ALVHVLSSAS IGNVSSVGVD STLNVVQDSV GQYVG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.1 % (1144 of 1203) | 94.1 % (558 of 593) | 96.0 % (457 of 476) | 96.3 % (129 of 134) |
Backbone | 94.7 % (708 of 748) | 92.9 % (248 of 267) | 95.2 % (340 of 357) | 96.8 % (120 of 124) |
Sidechain | 95.9 % (539 of 562) | 95.1 % (310 of 326) | 97.3 % (220 of 226) | 90.0 % (9 of 10) |
Aromatic | 100.0 % (44 of 44) | 100.0 % (22 of 22) | 100.0 % (22 of 22) | |
Methyl | 97.8 % (176 of 180) | 97.8 % (88 of 90) | 97.8 % (88 of 90) |
1. MiSp-CT
GSGNSVTSGG YGYGTSAAAG AGVAAGSYAG AVNRLSSAEA ASRVSSNIAA IASGGASALP SVISNIYSGV VASGVSSNEA LIQALLELLS ALVHVLSSAS IGNVSSVGVD STLNVVQDSV GQYVGSolvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Minor ampullate spidroin CT | [U-99% 13C; U-99% 15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 20 mM | |
4 | sodium azide | natural abundance | 0.03 % | |
5 | D2O | [U-100% 2H] | 8 % | |
6 | H2O | natural abundance | 92 % |