Structural insights into the DNA recognition and protein interaction domains reveal fundamental homologous DNA pairing properties of HOP2
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.2 % (815 of 979) | 81.3 % (418 of 514) | 84.6 % (314 of 371) | 88.3 % (83 of 94) |
Backbone | 93.4 % (467 of 500) | 93.1 % (161 of 173) | 93.9 % (230 of 245) | 92.7 % (76 of 82) |
Sidechain | 75.7 % (421 of 556) | 75.4 % (257 of 341) | 77.3 % (157 of 203) | 58.3 % (7 of 12) |
Aromatic | 0.0 % (0 of 66) | 0.0 % (0 of 33) | 0.0 % (0 of 33) | |
Methyl | 97.6 % (82 of 84) | 95.2 % (40 of 42) | 100.0 % (42 of 42) |
1. Hop2
MSKSRAEAAA GAPGIILRYL QEQNRPYSAQ DVFGNLQKEH GLGKAAVVKA LDQLAQEGKI KEKTYGKQKI YFADQNQFDT VSDASolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Hop2 | [U-99% 13C; U-99% 15N] | 6 mg/mL | |
2 | D2O | [U-99% 2H] | 5 % | |
3 | imidazole | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 120 mM | |
5 | glycerol | natural abundance | 5 % |