BMRB: 19623

GA-79-MBP cs-rosetta structures

Authors

He, Y., Chen, Y., Porter, L., Bryan, P., Orban, J.

Assembly

GA-79-MBP cs-rosetta structures

Entity

1. GA-79-MBP cs-rosetta structures (polymer, Thiol state: not present), 56 monomers, 6299.145 Da Detail

NGDKGYNGLA EAKEKAIKDL KIYGIGEHYI KLIEKAKQVA AVEDLKDEIL KAHDRF

Formula weight

6299.145 Da

Source organism

Streptococcus canis , Streptococcus dysgalactiae , Streptococcus equi , Finegoldia magna ATCC 29328

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 49.4 %, Completeness (bb): 97.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	49.4 % (332 of 672)	32.3 % (114 of 353)	62.7 % (163 of 260)	93.2 % (55 of 59)
Backbone	97.9 % (329 of 336)	95.7 % (112 of 117)	99.4 % (162 of 163)	98.2 % (55 of 56)
Sidechain	14.0 % (54 of 387)	0.8 % (2 of 236)	35.1 % (52 of 148)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 42)	0.0 % (0 of 21)	0.0 % (0 of 21)
Methyl	15.2 % (10 of 66)	6.1 % (2 of 33)	24.2 % (8 of 33)

1. entity

NGDKGYNGLA EAKEKAIKDL KIYGIGEHYI KLIEKAKQVA AVEDLKDEIL KAHDRF

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	GA-79-MBP	[U-100% 13C; U-100% 15N]		0.27 mM
2	potassium phosphate	natural abundance		100 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2MH8, Strand ID: A Detail

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Release date

2015-04-06

Citation

Subdomain interactions foster the design of two protein pairs with ∼80% sequence identity but different folds
Porter, L., He, Y., Chen, Y., Orban, J., Bryan, P.
Biophys. J. (2015), 108, 154-162, PubMed 25564862 , DOI 10.1016/j.bpj.2014.10.073 , Abstract

Related entities 1. GA-79-MBP cs-rosetta structures, : 1 : 106 entities Detail

Experiments performed 5 experiments Detail