BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.8 % (977 of 1100)	88.3 % (504 of 571)	90.4 % (387 of 428)	85.1 % (86 of 101)
Backbone	90.8 % (512 of 564)	91.3 % (178 of 195)	91.0 % (252 of 277)	89.1 % (82 of 92)
Sidechain	87.3 % (544 of 623)	86.7 % (326 of 376)	89.9 % (214 of 238)	44.4 % (4 of 9)
Aromatic	72.7 % (64 of 88)	72.7 % (32 of 44)	72.7 % (32 of 44)
Methyl	98.2 % (108 of 110)	98.2 % (54 of 55)	98.2 % (54 of 55)

1. TpsB4

MAHHHHHHVD DDDKMGGPTV LVKRFQLSGN RQFDDRRLLA LLHDLPGQEL NLSQLHAAAA RIGDFYQEKG YVLARAFLPA QEIQDGTVRI EVLEG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	TpsB4	[U-13C; U-15N]		1 mM
5	D2O	[U-100% 2H]		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.8 ppm	internal	direct	1.0

Referencing offset: 0.1 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MHJ, Strand ID: A Detail

Release date

2014-12-21

Citation

Structure-function analysis reveals a dual role of the Pseudomonas aeruginosa Tps4 two-partner secretion system
Garnett, J.A., Matthews, S.J.
Not known

Related entities 1. Pseudomonas aeruginosa Tps4 two-partner secretion system, : 1 : 9 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	TpsB4	[U-13C; U-15N]		1 mM
5	D2O	[U-100% 2H]		100 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	TpsB4	[U-13C; U-15N]		1 mM
5	D2O	[U-100% 2H]		100 %

4 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

5 3D HNCO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

6 3D HNCACB

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

7 3D HN(CO)CA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

8 3D H(CCO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

9 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	TpsB4	[U-13C; U-15N]		1 mM
5	D2O	[U-100% 2H]		100 %

10 3D 1H-13C NOESY aromatic

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	TpsB4	[U-13C; U-15N]		1 mM
5	D2O	[U-100% 2H]		100 %

11 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

12 3D HBHA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TpsB4	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %

nullKeywords biofilm, peptide, protein, secretion, two partner secreation

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BMRB: 19635

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Pseudomonas aeruginosa Tps4 two-partner secretion system, : 1 : 9 entities Detail

Experiments performed 12 experiments Detail

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