The solution NMR structure of maximin-4 in SDS micelles
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 96.6 % (144 of 149) | 96.6 % (144 of 149) |
Backbone | 98.3 % (58 of 59) | 98.3 % (58 of 59) |
Sidechain | 95.6 % (86 of 90) | 95.6 % (86 of 90) |
Aromatic | 100.0 % (4 of 4) | 100.0 % (4 of 4) |
Methyl | 100.0 % (22 of 22) | 100.0 % (22 of 22) |
1. maximin-4
GIGGVLLSAG KAALKGLAKV LAEKYANSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 2.8 mg lyophilized maximin-4 (MW =2,612) in 700 microliter 10 mM sodium-phosphate buffer (pH=5.0) containing 200 mM d25-SDS and 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | maximin-4 | natural abundance | 1.0 ~ 1.2 mM | |
2 | sodium-phosphate | natural abundance | 10 mM | |
3 | d25-sodium-dodecyl-sulfate | [U-100% 2H] | 200 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |