NMR structure of the I-V kissing-loop interaction of the Neurospora VS ribozyme
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 62.8 % (433 of 689) | 60.9 % (231 of 379) | 64.8 % (186 of 287) | 69.6 % (16 of 23) |
Suger, PO4 | 49.3 % (233 of 473) | 45.7 % (118 of 258) | 53.5 % (115 of 215) | |
Nucleobase | 92.6 % (200 of 216) | 93.4 % (113 of 121) | 98.6 % (71 of 72) | 69.6 % (16 of 23) |
Aromatic | 92.1 % (175 of 190) | 92.6 % (88 of 95) | 98.6 % (71 of 72) | 69.6 % (16 of 23) |
1. RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3')
GAGCAGCAUC GUCGGCUGCU CA2. RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3')
GCGGCAGUUG ACUACUGUCG CSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | natural abundance | 1.3 ~ 2.2 mM | |
2 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | [U-15N] | 1.3 ~ 2.2 mM | |
3 | TRIS | [U-2H] | 10 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | sodium azide | natural abundance | 0.05 mM | |
6 | magnesium chloride | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | natural abundance | 1.5 mM | |
8 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | [U-13C; U-15N] | 1.5 mM | |
9 | TRIS | [U-2H] | 10 mM | |
10 | sodium chloride | natural abundance | 50 mM | |
11 | sodium azide | natural abundance | 0.05 mM | |
12 | magnesium chloride | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | [U-15N] | 1.4 mM | |
14 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | natural abundance | 1.4 mM | |
15 | TRIS | [U-2H] | 10 mM | |
16 | sodium chloride | natural abundance | 50 mM | |
17 | sodium azide | natural abundance | 0.05 mM | |
18 | magnesium chloride | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | [U-13C; U-15N] | 1.7 mM | |
20 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | natural abundance | 1.7 mM | |
21 | TRIS | [U-2H] | 10 mM | |
22 | sodium chloride | natural abundance | 50 mM | |
23 | sodium azide | natural abundance | 0.05 mM | |
24 | magnesium chloride | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | [U-13C; U-15N]-Gua | 1.4 mM | |
26 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | natural abundance | 1.4 mM | |
27 | TRIS | [U-2H] | 10 mM | |
28 | sodium chloride | natural abundance | 50 mM | |
29 | sodium azide | natural abundance | 0.05 mM | |
30 | magnesium chloride | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details The labeling is actually [U-13C;U-15N]-Gua+Cyt, but is not in the choice
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
31 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | [U-13C; U-15N]-Gua | 1.4 mM | |
32 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | natural abundance | 1.4 mM | |
33 | TRIS | [U-2H] | 10 mM | |
34 | sodium chloride | natural abundance | 50 mM | |
35 | sodium azide | natural abundance | 0.05 mM | |
36 | magnesium chloride | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
37 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | [U-15N] | 1.4 mM | |
38 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | natural abundance | 1.4 mM | |
39 | TRIS | [U-2H] | 10 mM | |
40 | sodium chloride | natural abundance | 50 mM | |
41 | sodium azide | natural abundance | 0.05 mM | |
42 | magnesium chloride | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
43 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | natural abundance | 1.3 mM | |
44 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | [U-15N] | 1.3 mM | |
45 | TRIS | [U-2H] | 10 mM | |
46 | sodium chloride | natural abundance | 50 mM | |
47 | sodium azide | natural abundance | 0.05 mM | |
48 | magnesium chloride | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
49 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | [U-13C; U-15N] | 1.7 mM | |
50 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | natural abundance | 1.7 mM | |
51 | TRIS | [U-2H] | 10 mM | |
52 | sodium chloride | natural abundance | 50 mM | |
53 | sodium azide | natural abundance | 0.05 mM | |
54 | magnesium chloride | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
55 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | natural abundance | 1.5 mM | |
56 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | [U-13C; U-15N] | 1.5 mM | |
57 | TRIS | [U-2H] | 10 mM | |
58 | sodium chloride | natural abundance | 50 mM | |
59 | sodium azide | natural abundance | 0.05 mM | |
60 | magnesium chloride | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
61 | RNA (5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3') | [U-15N] | 0.2 ~ 0.3 mM | |
62 | RNA (5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3') | [U-15N] | 0.2 ~ 0.3 mM | |
63 | TRIS | [U-2H] | 10 mM | |
64 | sodium chloride | natural abundance | 50 mM | |
65 | sodium azide | natural abundance | 0.05 mM | |
66 | magnesium chloride | natural abundance | 5 mM | |
67 | Pf1 phage | natural abundance | 14.6-20.5 mg/mL |