BMRBj - BMREntryMaster

Found 1 Document (0.51 seconds)

BMRB: 19680

2MGZ

ASD-1 RRM domain

Authors

Amrane, S., Mackereth, C.D.

Assembly

ASD-1

Entity

1. ASD-1 (polymer, Thiol state: all free), 99 monomers, 10883.98 Da Detail

GAMGPVAPND RSTSSSSTDG PRRLHVSNIP FKYREPDLTA MFEKVGPVVD VEIIFNERGS KGFGFVTMQN PDDADRARAE FNGTTIEGRR VEVNLATQR

Formula weight

10883.98 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 49.9 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	49.9 % (552 of 1106)	32.5 % (188 of 578)	65.0 % (278 of 428)	86.0 % (86 of 100)
Backbone	92.1 % (534 of 580)	85.5 % (171 of 200)	96.2 % (277 of 288)	93.5 % (86 of 92)
Sidechain	17.0 % (105 of 616)	4.5 % (17 of 378)	38.3 % (88 of 230)	0.0 % (0 of 8)
Aromatic	0.0 % (0 of 72)	0.0 % (0 of 36)	0.0 % (0 of 36)
Methyl	12.0 % (11 of 92)	6.5 % (3 of 46)	17.4 % (8 of 46)

1. ASD-1

GAMGPVAPND RSTSSSSTDG PRRLHVSNIP FKYREPDLTA MFEKVGPVVD VEIIFNERGS KGFGFVTMQN PDDADRARAE FNGTTIEGRR VEVNLATQR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	ASD-1	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	300 mM
4	D2O	[U-2H]	10 %
5	H2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	ASD-1	[U-99% 15N]	3.28 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	300 mM
9	D2O	[U-2H]	10 %
10	H2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Release date

2014-08-28

Citation

Backbone-independent nucleic acid binding by splicing factor SUP-12 reveals key aspects of molecular recognition
Amrane, S., Rebora, K., Zniber, I., Dupuy, D., Mackereth, C.D.
Nat. Commun. (2014), 5, 4595-4595, PubMed 25183497 , DOI 10.1038/ncomms5595 , Abstract

Related entities 1. ASD-1, : 1 : 153 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr19680_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr19680_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	534		97.0 (chain: 1, length: 99)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 99, Sequence coverage: 97.0%
- Total number of assignments
  - ¹³C chemical shifts: 277
  - ¹H chemical shifts: 171
  - ¹⁵N chemical shifts: 86
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	428	277	64.7
¹H chemical shifts	578	171	29.6
¹⁵N chemical shifts	110	86	78.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	198	190	96.0
¹H chemical shifts	200	171	85.5
¹⁵N chemical shifts	92	86	93.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	230	87	37.8
¹H chemical shifts	378	0	0.0
¹⁵N chemical shifts	18	0	0.0

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Found 1 Document (0.51 seconds)

BMRB: 19680

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. ASD-1, : 1 : 153 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail