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BMRB: 19699

2MIZ

Structure of the m04/gp34 mouse Cytomegalovirus Immunoevasin core domain

Authors

Sgourakis, N.G., Natarajan, K., Margulies, D.H., Bax, A.

Assembly

m04/gp34 mouse Cytomegalovirus Immunoevasin core domain

Entity

1. m04/gp34 mouse Cytomegalovirus Immunoevasin core domain (polymer, Thiol state: all disulfide bound), 156 monomers, 17473.66 Da Detail

RHSLGCYFKG INPTKVPSSD PRTVLKCTLP DVKVNASWTL EWVVVNLHTS VDVTSYYESS PNSEPRFLRA ILNFTPMHGL RTKNLLKVKD GFQVDNSTDN GNGGNLYVYP NATTGSADSV RCRLRMCPWT SNSKMTAPDE EMLRKMSEVL NLPNYG

Formula weight

17473.66 Da

Source organism

Maize chlorotic mottle virus

Exptl. method

solution NMR

Refine. method

RASREC

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 40.7 %, Completeness (bb): 77.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	40.7 % (731 of 1797)	18.6 % (174 of 934)	59.1 % (414 of 700)	87.7 % (143 of 163)
Backbone	77.2 % (706 of 914)	48.4 % (150 of 310)	90.0 % (413 of 459)	98.6 % (143 of 145)
Sidechain	14.9 % (153 of 1030)	3.8 % (24 of 624)	33.2 % (129 of 388)	0.0 % (0 of 18)
Aromatic	2.9 % (4 of 136)	5.9 % (4 of 68)	0.0 % (0 of 65)	0.0 % (0 of 3)
Methyl	3.1 % (5 of 160)	1.3 % (1 of 80)	5.0 % (4 of 80)

1. entity

RHSLGCYFKG INPTKVPSSD PRTVLKCTLP DVKVNASWTL EWVVVNLHTS VDVTSYYESS PNSEPRFLRA ILNFTPMHGL RTKNLLKVKD GFQVDNSTDN GNGGNLYVYP NATTGSADSV RCRLRMCPWT SNSKMTAPDE EMLRKMSEVL NLPNYG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20mM mM
2	D2O	[U-99% 2H]	5 %
3	sodium chloride	natural abundance	50 mM
4	gp34/m04	[U-13C; U-15N; U-2H]	0.2 ~ 0.5 mM
5	H2O	natural abundance

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	20mM mM
7	D2O	[U-99% 2H]	5 %
8	sodium chloride	natural abundance	50 mM
9	gp34/m04	[U-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
10	H2O	natural abundance

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
11	sodium phosphate	natural abundance	20mM mM
12	D2O	[U-99% 2H]	5 %
13	sodium chloride	natural abundance	50 mM
14	gp34/m04	[U-15N; U-2H]	0.2 ~ 0.5 mM
15	Ac/Bis-Ac	natural abundance	5.5 %
16	DADMAC	natural abundance	20 %
17	H2O	natural abundance

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	sodium phosphate	natural abundance	20mM mM
19	D2O	[U-99% 2H]	5 %
20	sodium chloride	natural abundance	200 mM
21	gp34/m04	[U-15N; U-2H]	0.2 ~ 0.5 mM
22	Pf1 phage	natural abundance	7.5 %
23	H2O	natural abundance

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	sodium phosphate	natural abundance	20mM mM
25	D2O	[U-99% 2H]	5 %
26	sodium chloride	natural abundance	50 mM
27	gp34/m04	[ILVmethyl-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
28	H2O	natural abundance

Sample #6

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
29	sodium phosphate	natural abundance	20mM mM
30	D2O	[U-99% 2H]	5 %
31	sodium chloride	natural abundance	50 mM
32	gp34/m04	[U-15N; U-2H]	0.2 ~ 0.5 mM
33	H2O	natural abundance

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.72 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.72 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.49 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MIZ, Strand ID: A Detail

Release date

2014-01-02

Citation

The murine Cytomegalovirus gp34/m04 protein contains a core domain with novel structure revealed by solution NMR
Sgourakis, N.G., Natarajan, K., Margulies, D.H., Bax, A.
Not known

Related entities 1. m04/gp34 mouse Cytomegalovirus Immunoevasin core domain, : 1 : 1 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N TROSY-HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20mM mM
2	D2O	[U-99% 2H]	5 %
3	sodium chloride	natural abundance	50 mM
4	gp34/m04	[U-13C; U-15N; U-2H]	0.2 ~ 0.5 mM
5	H2O	natural abundance

2 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20mM mM
2	D2O	[U-99% 2H]	5 %
3	sodium chloride	natural abundance	50 mM
4	gp34/m04	[U-13C; U-15N; U-2H]	0.2 ~ 0.5 mM
5	H2O	natural abundance

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20mM mM
2	D2O	[U-99% 2H]	5 %
3	sodium chloride	natural abundance	50 mM
4	gp34/m04	[U-13C; U-15N; U-2H]	0.2 ~ 0.5 mM
5	H2O	natural abundance

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20mM mM
2	D2O	[U-99% 2H]	5 %
3	sodium chloride	natural abundance	50 mM
4	gp34/m04	[U-13C; U-15N; U-2H]	0.2 ~ 0.5 mM
5	H2O	natural abundance

5 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20mM mM
2	D2O	[U-99% 2H]	5 %
3	sodium chloride	natural abundance	50 mM
4	gp34/m04	[U-13C; U-15N; U-2H]	0.2 ~ 0.5 mM
5	H2O	natural abundance

6 2D 1H-15N ARTSY

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	sodium phosphate	natural abundance	20mM mM
19	D2O	[U-99% 2H]	5 %
20	sodium chloride	natural abundance	200 mM
21	gp34/m04	[U-15N; U-2H]	0.2 ~ 0.5 mM
22	Pf1 phage	natural abundance	7.5 %
23	H2O	natural abundance

7 2D 1H-15N ARTSY

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
11	sodium phosphate	natural abundance	20mM mM
12	D2O	[U-99% 2H]	5 %
13	sodium chloride	natural abundance	50 mM
14	gp34/m04	[U-15N; U-2H]	0.2 ~ 0.5 mM
15	Ac/Bis-Ac	natural abundance	5.5 %
16	DADMAC	natural abundance	20 %
17	H2O	natural abundance

8 3D 1H-13C NOESY aliphatic (HCH)

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	sodium phosphate	natural abundance	20mM mM
25	D2O	[U-99% 2H]	5 %
26	sodium chloride	natural abundance	50 mM
27	gp34/m04	[ILVmethyl-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
28	H2O	natural abundance

9 3D 1H-13C NOESY aliphatic (CCH)

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	sodium phosphate	natural abundance	20mM mM
25	D2O	[U-99% 2H]	5 %
26	sodium chloride	natural abundance	50 mM
27	gp34/m04	[ILVmethyl-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
28	H2O	natural abundance

10 2D 1H-13C HMQC methyl

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	sodium phosphate	natural abundance	20mM mM
25	D2O	[U-99% 2H]	5 %
26	sodium chloride	natural abundance	50 mM
27	gp34/m04	[ILVmethyl-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
28	H2O	natural abundance

11 3D 1H-15N NOESY (HCH)

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	sodium phosphate	natural abundance	20mM mM
25	D2O	[U-99% 2H]	5 %
26	sodium chloride	natural abundance	50 mM
27	gp34/m04	[ILVmethyl-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
28	H2O	natural abundance

12 3D 1H-15N NOESY (HCN)

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	sodium phosphate	natural abundance	20mM mM
25	D2O	[U-99% 2H]	5 %
26	sodium chloride	natural abundance	50 mM
27	gp34/m04	[ILVmethyl-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
28	H2O	natural abundance

13 3D 1H-15N NOESY (HNH)

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
29	sodium phosphate	natural abundance	20mM mM
30	D2O	[U-99% 2H]	5 %
31	sodium chloride	natural abundance	50 mM
32	gp34/m04	[U-15N; U-2H]	0.2 ~ 0.5 mM
33	H2O	natural abundance

14 3D 1H-15N NOESY (NNH)

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
29	sodium phosphate	natural abundance	20mM mM
30	D2O	[U-99% 2H]	5 %
31	sodium chloride	natural abundance	50 mM
32	gp34/m04	[U-15N; U-2H]	0.2 ~ 0.5 mM
33	H2O	natural abundance

15 SIM-HMCM(CGCBCA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	20mM mM
7	D2O	[U-99% 2H]	5 %
8	sodium chloride	natural abundance	50 mM
9	gp34/m04	[U-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
10	H2O	natural abundance

16 HMCM(CG)CBCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	20mM mM
7	D2O	[U-99% 2H]	5 %
8	sodium chloride	natural abundance	50 mM
9	gp34/m04	[U-13C; U-15N; U-2H; ILVmethyl-1H]	0.2 ~ 0.5 mM
10	H2O	natural abundance

nullKeywords ILV labelling, Immunoglobulin-like fold, MHC class-I regulation, Natural Killer decoy, Residual Dipolar Couplings, Rosetta modelling, missing-self

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Found 1 Document (0.75 seconds)

BMRB: 19699

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Sample #6

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. m04/gp34 mouse Cytomegalovirus Immunoevasin core domain, : 1 : 1 entities Detail

Experiments performed 16 experiments Detail

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