Backbone 1H, 13C, and 15N Chemical Shift Assignments and structure of Iron-sulfur cluster binding protein from Ehrlichia chaffeensis
MPLITFISPD GSRKTYEAYD GETLLSLAHR NNVDLEGACE GSLACSTCHV IIDPSWYDIV EQHNEISDEE NDMLDLAFGL TDTSRLGCQI ILTKELDGLC VILPTETRNI SFVKNS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.6 % (1006 of 1296) | 74.7 % (496 of 664) | 79.6 % (407 of 511) | 85.1 % (103 of 121) |
Backbone | 88.2 % (607 of 688) | 87.2 % (205 of 235) | 88.9 % (303 of 341) | 88.4 % (99 of 112) |
Sidechain | 69.2 % (496 of 717) | 67.8 % (291 of 429) | 72.0 % (201 of 279) | 44.4 % (4 of 9) |
Aromatic | 33.3 % (26 of 78) | 64.1 % (25 of 39) | 0.0 % (0 of 38) | 100.0 % (1 of 1) |
Methyl | 78.5 % (113 of 144) | 75.0 % (54 of 72) | 81.9 % (59 of 72) |
1. Iron-sulfur cluster binding protein
MPLITFISPD GSRKTYEAYD GETLLSLAHR NNVDLEGACE GSLACSTCHV IIDPSWYDIV EQHNEISDEE NDMLDLAFGL TDTSRLGCQI ILTKELDGLC VILPTETRNI SFVKNSSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Iron-sulfur cluster binding protein | [U-95% 15N] | 1.2 mM | |
2 | Iron-sulfur cluster binding protein | [U-95% 13C; U-95% 15N] | 1.1 mM |