Solution structure of tandem RRM domains of cytoplasmic polyadenylation element binding protein 1 (CPEB1) in free state
MTWSGQLPPR NYKNPIYSCK VFLGGVPWDI TEAGLVNTFR VFGSLSVEWP GKDGKHPRCP PKGYVYLVFE LEKSVRSLLQ ACSHDPLSPD GLSEYYFKMS SRRMRCKEVQ VIPWVLADSN FVRSPSQRLD PSRTVFVGAL HGMLNAEALA AILNDLFGGV VYAGIDTDKH KYPIGSGRVT FNNQRSYLKA VSAAFVEIKT TKFTKKVQID PYLEDSL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 74.1 % (1903 of 2569) | 79.3 % (1060 of 1336) | 62.2 % (630 of 1013) | 96.8 % (213 of 220) |
Backbone | 73.3 % (933 of 1272) | 85.5 % (372 of 435) | 57.5 % (365 of 635) | 97.0 % (196 of 202) |
Sidechain | 76.2 % (1142 of 1498) | 76.4 % (688 of 901) | 75.5 % (437 of 579) | 94.4 % (17 of 18) |
Aromatic | 73.6 % (187 of 254) | 88.2 % (112 of 127) | 57.7 % (71 of 123) | 100.0 % (4 of 4) |
Methyl | 91.5 % (214 of 234) | 90.6 % (106 of 117) | 92.3 % (108 of 117) |
1. CPEB1RRM12
MTWSGQLPPR NYKNPIYSCK VFLGGVPWDI TEAGLVNTFR VFGSLSVEWP GKDGKHPRCP PKGYVYLVFE LEKSVRSLLQ ACSHDPLSPD GLSEYYFKMS SRRMRCKEVQ VIPWVLADSN FVRSPSQRLD PSRTVFVGAL HGMLNAEALA AILNDLFGGV VYAGIDTDKH KYPIGSGRVT FNNQRSYLKA VSAAFVEIKT TKFTKKVQID PYLEDSLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CPEB1RRM12 | [U-100% 15N] | 0.4 ~ 0.6 mM | |
2 | sodium chloride | natural abundance | 100 mM | |
3 | DTT | natural abundance | 1 mM | |
4 | magnesium sulphate | natural abundance | 1 mM | |
5 | sodium phosphate | natural abundance | 50 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | CPEB1RRM12 | [U-100% 13C; U-100% 15N] | 0.4 ~ 0.6 mM | |
9 | sodium chloride | natural abundance | 100 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | magnesium sulphate | natural abundance | 1 mM | |
12 | sodium phosphate | natural abundance | 50 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | CPEB1RRM12 | [U-100% 13C; U-100% 15N; U-100% 2H] | 0.4 ~ 0.6 mM | |
16 | sodium chloride | natural abundance | 100 mM | |
17 | DTT | natural abundance | 1 mM | |
18 | magnesium sulphate | natural abundance | 1 mM | |
19 | sodium phosphate | natural abundance | 50 mM | |
20 | H2O | natural abundance | 90 % | |
21 | D2O | natural abundance | 10 % |