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BMRB: 19779

4CPG

Solution structure of the SGTA N-terminal domain

Authors

Darby, J.F., Krysztofinska, E.M., Simpson, P.J., Isaacson, R.

Assembly

Solution structure of the SGTA N-terminal domain

Entity

1. Solution structure of the SGTA N-terminal domain (polymer, Thiol state: all free), 83 monomers, 9229.144 × 2 Da Detail

MAHHHHHHVD DDDKMDNKKR LAYAIIQFLH DQLRHGGLSS DAQESLEVAI QCLETAFGVT VEDSDLALPQ TLPEIFEAAA TGK

Total weight

18458.287 Da

Max. entity weight

9229.144 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.3 %, Completeness: 82.2 %, Completeness (bb): 82.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.2 % (764 of 929)	81.3 % (387 of 476)	82.8 % (303 of 366)	85.1 % (74 of 87)
Backbone	82.8 % (409 of 494)	82.1 % (138 of 168)	82.9 % (203 of 245)	84.0 % (68 of 81)
Sidechain	81.9 % (421 of 514)	80.8 % (249 of 308)	83.0 % (166 of 200)	100.0 % (6 of 6)
Aromatic	67.1 % (47 of 70)	68.6 % (24 of 35)	65.7 % (23 of 35)
Methyl	92.7 % (89 of 96)	91.7 % (44 of 48)	93.8 % (45 of 48)

1. SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA

MAHHHHHHVD DDDKMDNKKR LAYAIIQFLH DQLRHGGLSS DAQESLEVAI QCLETAFGVT VEDSDLALPQ TLPEIFEAAA TGK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.000, Details 13C,15N SGTA 0.75mM

#	Name	Isotope labeling	Type	Concentration
1	SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA	[U-13C; U-15N]		0.75 mM

Sample #2

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.000, Details 13C,15N SGTA 0.75mM

#	Name	Isotope labeling	Type	Concentration
2	SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA	[U-13C; U-15N]		0.75 mM

Sample #3

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.000, Details 13C-15N SGTA 0.4mM, 12C-14N SGTA 0.4mM

#	Name	Isotope labeling	Type	Concentration
3	SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA	[U-13C; U-15N]		0.4 mM
4	SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA	natural abundance		0.4 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.77 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 4CPG, Strand ID: A, B Detail

Release date

2014-11-30

Citation

Solution structure of the SGTA dimerisation domain and investigation of its interactions with the ubiquitin-like domains of BAG6 and UBL4A
Darby, J.F., Krysztofinska, E.M., Simpson, P.J., Simon, A.C., Sriskandarajah, N., Bishop, D.S., Hale, L.R., Alfano, C., Conte, M.R., High, S., Isaacson, R.
PLoS One (2014), 9, e113281-e113281, PubMed 25415308 , DOI 10.1371/journal.pone.0113281 , Abstract

Related entities 1. Solution structure of the SGTA N-terminal domain, : 1 : 2 : 14 entities Detail

Interaction partners 1. Solution structure of the SGTA N-terminal domain, : 120 interactors Detail

More details for 120 interactors identified by 15 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC