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BMRB: 19789

2MKP

N domain of cardiac troponin C bound to the switch fragment of fast skeletal troponin I at pH 6

Authors

Robertson, I.M., Pineda-Sanabria, S.E., Holmes, P.C., Sykes, B.D.

Assembly

N domain of cardiac troponin C bound to the switch fragment of fast skeletal troponin I

Entity

1. cNTnC (polymer), 89 monomers, 10062.23 Da Detail

MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDS

2. sTnI(115-131) (polymer, Thiol state: not present), 17 monomers, 1857.248 Da Detail

RMSADAMLKA LLGSKHK

3. CALCIUM ION (non-polymer), 40.078 Da

Total weight

11959.557 Da

Max. entity weight

10062.23 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 79.2 %, Completeness: 35.1 %, Completeness (bb): 41.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	35.1 % (425 of 1212)	36.6 % (231 of 632)	23.8 % (112 of 470)	74.5 % (82 of 110)
Backbone	41.6 % (263 of 632)	55.3 % (120 of 217)	19.6 % (61 of 311)	78.8 % (82 of 104)
Sidechain	28.1 % (191 of 679)	26.7 % (111 of 415)	31.0 % (80 of 258)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 62)	0.0 % (0 of 31)	0.0 % (0 of 31)
Methyl	80.8 % (84 of 104)	80.8 % (42 of 52)	80.8 % (42 of 52)

1. cNTnC

MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDS

2. sTnI(115-131)

RMSADAMLKA LLGSKHK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.00

#	Name	Isotope labeling	Concentration
1	cNTnC	[U-95% 13C; U-95% 15N]	0.2 ~ 0.3 mM
2	sTnI(115-131)	natural abundance	2.0 ~ 2.5 mM
3	DSS	natural abundance	0.25 mM
4	potassium chloride	natural abundance	100 mM
5	imidazole	natural abundance	10 mM
6	Calcium	natural abundance	10 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Chem. Shift Complete2

Sequence coverage: 23.6 %, Completeness: 23.6 %, Completeness (bb): 27.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	23.6 % (573 of 2424)	26.3 % (333 of 1264)	15.6 % (147 of 940)	42.3 % (93 of 220)
Backbone	27.8 % (351 of 1264)	38.2 % (166 of 434)	14.8 % (92 of 622)	44.7 % (93 of 208)
Sidechain	19.2 % (261 of 1358)	20.1 % (167 of 830)	18.2 % (94 of 516)	0.0 % (0 of 12)
Aromatic	0.8 % (1 of 124)	1.6 % (1 of 62)	0.0 % (0 of 62)
Methyl	45.7 % (95 of 208)	50.0 % (52 of 104)	41.3 % (43 of 104)

1. cNTnC

MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDS

2. sTnI(115-131)

RMSADAMLKA LLGSKHK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.00

#	Name	Isotope labeling	Concentration
1	cNTnC	[U-95% 13C; U-95% 15N]	0.2 ~ 0.3 mM
2	sTnI(115-131)	natural abundance	2.0 ~ 2.5 mM
3	DSS	natural abundance	0.25 mM
4	potassium chloride	natural abundance	100 mM
5	imidazole	natural abundance	10 mM
6	Calcium	natural abundance	10 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2MKP, Strand ID: C, I Detail

Release date

2014-02-10

Citation

Conformation of the critical pH sensitive region of troponin depends upon a single residue in troponin I
Robertson, I.M., Pineda-Sanabria, S.E., Holmes, P.C., Sykes, B.D.
Arch. Biochem. Biophys. (2014), 552-553, 40-49, PubMed 24333682 , DOI 10.1016/j.abb.2013.12.003 , Abstract

Related entities 1. cNTnC, : 3 : 96 entities Detail

Related entities 2. sTnI(115-131), : 1 : 7 : 20 : 31 entities Detail

Interaction partners 2. sTnI(115-131), : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC