NMR structure of B25-(alpha, beta)-dehydro-phenylalanine insulin
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.9 % (466 of 569) | 94.5 % (275 of 291) | 66.8 % (149 of 223) | 76.4 % (42 of 55) |
Backbone | 73.2 % (218 of 298) | 93.2 % (96 of 103) | 58.9 % (86 of 146) | 73.5 % (36 of 49) |
Sidechain | 91.2 % (289 of 317) | 95.2 % (179 of 188) | 84.6 % (104 of 123) | 100.0 % (6 of 6) |
Aromatic | 82.1 % (46 of 56) | 100.0 % (28 of 28) | 64.3 % (18 of 28) | |
Methyl | 96.4 % (54 of 56) | 100.0 % (28 of 28) | 92.9 % (26 of 28) |
1. entity 1
GIVEQCCTSI CSLYQLENYC N2. entity 2
FVNQHLCGSD LVEALYLVCG ERGFXYTKPTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-13C; U-15N] | 0.5 mM | |
2 | entity_2 | [U-13C; U-15N] | 0.5 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | natural abundance | 0.5 mM | |
6 | entity_2 | natural abundance | 0.5 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |