A Mechanism for the cAMP-Dependent Auto-Inhibition of HCN
NSEEIINFNC RKLVASMPLF ANADPNFVTS MLTKLRFEVF QPGDYIIREG TIGKKMYFIQ HGVVSVLTKG NKETKLADGS YFGEICLLTR GRRTASVRAD TYCRLYSLSV DNFNEVLEEY PMMRRAFETV A
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.7 % (1206 of 1553) | 75.4 % (608 of 806) | 78.2 % (477 of 610) | 88.3 % (121 of 137) |
Backbone | 93.2 % (725 of 778) | 93.6 % (249 of 266) | 92.2 % (355 of 385) | 95.3 % (121 of 127) |
Sidechain | 66.4 % (596 of 898) | 66.5 % (359 of 540) | 68.1 % (237 of 348) | 0.0 % (0 of 10) |
Aromatic | 11.3 % (16 of 142) | 12.7 % (9 of 71) | 9.9 % (7 of 71) | |
Methyl | 74.7 % (109 of 146) | 67.1 % (49 of 73) | 82.2 % (60 of 73) |
1. Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
NSEEIINFNC RKLVASMPLF ANADPNFVTS MLTKLRFEVF QPGDYIIREG TIGKKMYFIQ HGVVSVLTKG NKETKLADGS YFGEICLLTR GRRTASVRAD TYCRLYSLSV DNFNEVLEEY PMMRRAFETV ASolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DTT | natural abundance | 1 mM | |
2 | MES | natural abundance | 20 mM | |
3 | potassium chloride | natural abundance | 100 mM | |
4 | EDTA | natural abundance | 2 mM | |
5 | EGTA | natural abundance | 2 mM | |
6 | sodium azide | natural abundance | 0.02 % | |
7 | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 | [U-13C; U-15N] | 0.5 mM | |
8 | D2O | natural abundance | 95 % | |
9 | H2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | DTT | natural abundance | 1 mM | |
11 | MES | natural abundance | 20 mM | |
12 | potassium chloride | natural abundance | 100 mM | |
13 | EDTA | natural abundance | 1 mM | |
14 | sodium azide | natural abundance | 0.02 % | |
15 | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 | [U-15N] | 0.2 mM | |
16 | Pf1 phage | natural abundance | 6 mg/mL | |
17 | D2O | natural abundance | 95 % | |
18 | H2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | DTT | natural abundance | 1 mM | |
20 | MES | natural abundance | 20 mM | |
21 | potassium chloride | natural abundance | 100 mM | |
22 | EDTA | natural abundance | 1 mM | |
23 | sodium azide | natural abundance | 0.02 % | |
24 | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 | [U-15N] | 0.2 mM | |
25 | D2O | natural abundance | 95 % | |
26 | H2O | natural abundance | 5 % |