Solution structure of MBD4 methyl-cytosine binding domain bound to methylated DNA
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 72.0 % (742 of 1031) | 61.0 % (381 of 625) | 87.4 % (291 of 333) | 95.9 % (70 of 73) |
Backbone | 73.6 % (402 of 546) | 50.6 % (137 of 271) | 96.6 % (200 of 207) | 95.6 % (65 of 68) |
Sidechain | 72.4 % (398 of 550) | 67.8 % (240 of 354) | 80.1 % (153 of 191) | 100.0 % (5 of 5) |
Aromatic | 34.4 % (44 of 128) | 30.5 % (25 of 82) | 40.0 % (18 of 45) | 100.0 % (1 of 1) |
Methyl | 79.4 % (50 of 63) | 75.8 % (25 of 33) | 83.3 % (25 of 30) |
1. MBD4mbd
GSTECRKSVP CGWERVVKQR LFGKTAGRFD VYFISPQGLK FRSKSSLANY LHKNGETSLK PEDFDFTVLS K2. DNAfor
GGATXGGCTC3. DNArev
GAGCXGATCCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MBD4mbd | [U-99% 13C; U-99% 15N] | 0.2 ~ 1.0 mM | |
2 | DNAfor | natural abundance | 0.2 ~ 1.0 mM | |
3 | DNArev | natural abundance | 0.2 ~ 1.0 mM | |
4 | Na2PO4 | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | MBD4mbd | [U-13C; U-15N; U-2H] | 0.2 ~ 1.0 mM | |
6 | DNAfor | natural abundance | 0.2 ~ 1.0 mM | |
7 | DNArev | natural abundance | 0.2 ~ 1.0 mM | |
8 | Pf1 phage | natural abundance | 12 mg/mL | |
9 | Na2PO4 | natural abundance | 10 mM |
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 43.8 % (904 of 2062) | 38.5 % (481 of 1250) | 51.4 % (342 of 666) | 55.5 % (81 of 146) |
Backbone | 44.5 % (486 of 1092) | 32.7 % (177 of 542) | 56.5 % (234 of 414) | 55.1 % (75 of 136) |
Sidechain | 44.3 % (487 of 1100) | 42.4 % (300 of 708) | 47.4 % (181 of 382) | 60.0 % (6 of 10) |
Aromatic | 27.0 % (69 of 256) | 27.4 % (45 of 164) | 25.6 % (23 of 90) | 50.0 % (1 of 2) |
Methyl | 49.2 % (62 of 126) | 48.5 % (32 of 66) | 50.0 % (30 of 60) |
1. MBD4mbd
GSTECRKSVP CGWERVVKQR LFGKTAGRFD VYFISPQGLK FRSKSSLANY LHKNGETSLK PEDFDFTVLS K2. DNAfor
GGATXGGCTC3. DNArev
GAGCXGATCCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MBD4mbd | [U-99% 13C; U-99% 15N] | 0.2 ~ 1.0 mM | |
2 | DNAfor | natural abundance | 0.2 ~ 1.0 mM | |
3 | DNArev | natural abundance | 0.2 ~ 1.0 mM | |
4 | Na2PO4 | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | MBD4mbd | [U-13C; U-15N; U-2H] | 0.2 ~ 1.0 mM | |
6 | DNAfor | natural abundance | 0.2 ~ 1.0 mM | |
7 | DNArev | natural abundance | 0.2 ~ 1.0 mM | |
8 | Pf1 phage | natural abundance | 12 mg/mL | |
9 | Na2PO4 | natural abundance | 10 mM |
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 33.1 % (1024 of 3093) | 29.8 % (559 of 1875) | 37.4 % (374 of 999) | 41.6 % (91 of 219) |
Backbone | 33.7 % (552 of 1638) | 26.1 % (212 of 813) | 41.2 % (256 of 621) | 41.2 % (84 of 204) |
Sidechain | 33.3 % (549 of 1650) | 32.3 % (343 of 1062) | 34.7 % (199 of 573) | 46.7 % (7 of 15) |
Aromatic | 20.8 % (80 of 384) | 22.8 % (56 of 246) | 17.0 % (23 of 135) | 33.3 % (1 of 3) |
Methyl | 35.4 % (67 of 189) | 34.3 % (34 of 99) | 36.7 % (33 of 90) |
1. MBD4mbd
GSTECRKSVP CGWERVVKQR LFGKTAGRFD VYFISPQGLK FRSKSSLANY LHKNGETSLK PEDFDFTVLS K2. DNAfor
GGATXGGCTC3. DNArev
GAGCXGATCCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MBD4mbd | [U-99% 13C; U-99% 15N] | 0.2 ~ 1.0 mM | |
2 | DNAfor | natural abundance | 0.2 ~ 1.0 mM | |
3 | DNArev | natural abundance | 0.2 ~ 1.0 mM | |
4 | Na2PO4 | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | MBD4mbd | [U-13C; U-15N; U-2H] | 0.2 ~ 1.0 mM | |
6 | DNAfor | natural abundance | 0.2 ~ 1.0 mM | |
7 | DNArev | natural abundance | 0.2 ~ 1.0 mM | |
8 | Pf1 phage | natural abundance | 12 mg/mL | |
9 | Na2PO4 | natural abundance | 10 mM |