Structural insights of TM domain of LAMP-2A in DPC micelles
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 78.4 % (373 of 476) | 73.8 % (175 of 237) | 81.6 % (160 of 196) | 88.4 % (38 of 43) |
Backbone | 90.0 % (225 of 250) | 89.7 % (78 of 87) | 89.3 % (109 of 122) | 92.7 % (38 of 41) |
Sidechain | 68.9 % (182 of 264) | 64.7 % (97 of 150) | 75.9 % (85 of 112) | 0.0 % (0 of 2) |
Aromatic | 43.1 % (25 of 58) | 41.4 % (12 of 29) | 44.8 % (13 of 29) | |
Methyl | 82.9 % (58 of 70) | 82.9 % (29 of 35) | 82.9 % (29 of 35) |
1. TM domain of LAMP2A
SADDDNFLVP IAVGAALAGV LILVLLAYFI GLKHHHAGYE QFSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.3, Details (15N) TM-LAMP2A
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TM domain of LAMP2A | [U-99% 15N] | 0.6 (±0.1) mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.3, Details (15N/13C) TM-LAMP2A
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | TM domain of LAMP2A | [U-99% 13C; U-99% 15N] | 0.6 (±0.1) mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.3, Details (15N/13C) TM-LAMP2A
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | TM domain of LAMP2A | [U-99% 13C; U-99% 15N] | 0.6 (±0.1) mM | |
8 | D2O | natural abundance | 100 % |