Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)
MDFKAIAQQT AQEVLGYNRD TSGWKVVKTS KKITVSSKAS RKFHGNLYRV EGIIPESPAK LSDFLYQTGD RITWDKSLQV YNMVHRIDSD TFICHTITQS FAVGSISPRD FIDLVYIKRY EGNMNIISSK SVDFPEYPPS SNYIRGYNHP CGFVCSPMEE NPAYSKLVMF VQTEMRGKLS PSIIEKTMPS NLVNFILNAK DGIKAHRTPS RRGFHHNSHS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.1 % (2249 of 2611) | 85.0 % (1159 of 1363) | 86.7 % (883 of 1019) | 90.4 % (207 of 229) |
Backbone | 96.1 % (1245 of 1296) | 96.1 % (423 of 440) | 95.5 % (619 of 648) | 97.6 % (203 of 208) |
Sidechain | 79.3 % (1207 of 1523) | 79.7 % (736 of 923) | 80.7 % (467 of 579) | 19.0 % (4 of 21) |
Aromatic | 30.1 % (74 of 246) | 30.9 % (38 of 123) | 28.1 % (34 of 121) | 100.0 % (2 of 2) |
Methyl | 98.6 % (207 of 210) | 98.1 % (103 of 105) | 99.0 % (104 of 105) |
1. StAR D6
MDFKAIAQQT AQEVLGYNRD TSGWKVVKTS KKITVSSKAS RKFHGNLYRV EGIIPESPAK LSDFLYQTGD RITWDKSLQV YNMVHRIDSD TFICHTITQS FAVGSISPRD FIDLVYIKRY EGNMNIISSK SVDFPEYPPS SNYIRGYNHP CGFVCSPMEE NPAYSKLVMF VQTEMRGKLS PSIIEKTMPS NLVNFILNAK DGIKAHRTPS RRGFHHNSHSSolvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.2514495 |
1H | water | proton | 4.66 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.2514495 |
1H | water | proton | 4.66 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.2514495 |
1H | water | proton | 4.66 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.2514495 |
1H | water | proton | 4.66 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | internal | indirect | 0.1013291 |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Varian UnityInova - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.150 K, pH 7.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StAR_D6 | [U-100% 13C; U-100% 15N] | 1 mM |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_19952_2mou.nef |
Input source #2: Coordindates | 2mou.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEYPPSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLSPSIIEKTMPSNLVNFILNAK |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEYPPSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLSPSIIEKTMPSNLVNFILNAK -------210-------220 DGIKAHRTPSRRGFHHNSHS |||||||||||||||||||| DGIKAHRTPSRRGFHHNSHS
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 220 | 0 | 0 | 100.0 |
Content subtype: combined_19952_2mou.nef
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEYPPSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLSPSIIEKTMPSNLVNFILNAK ||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||| FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEY.PSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLS.SIIEKTMPSNLVNFILNAK -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 -------210-------220 DGIKAHRTPSRRGFHHNSHS |||||||||||||| ||| DGIKAHRTPSRRGF.HNS -------210--------
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1363 | 1142 | 83.8 |
13C chemical shifts | 1019 | 878 | 86.2 |
15N chemical shifts | 241 | 201 | 83.4 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 440 | 418 | 95.0 |
13C chemical shifts | 440 | 413 | 93.9 |
15N chemical shifts | 208 | 199 | 95.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 923 | 724 | 78.4 |
13C chemical shifts | 579 | 465 | 80.3 |
15N chemical shifts | 33 | 2 | 6.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 112 | 106 | 94.6 |
13C chemical shifts | 112 | 106 | 94.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 123 | 36 | 29.3 |
13C chemical shifts | 121 | 32 | 26.4 |
15N chemical shifts | 2 | 2 | 100.0 |
Distance restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEYPPSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLSPSIIEKTMPSNLVNFILNAK ||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||| FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEY.PSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLS.SIIEKTMPSNLVNFILNAK -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 -------210-------220 DGIKAHRTPSRRGFHHNSHS |||||||||||||| || DGIKAHRTPSRRGF.HN -------210-------
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS | || | ||||||||| | ||| |||| || ||||||| | || ||||| ||| || |||||| | ||||| | ..F..IA.Q.AQEVLGYNR.T......KTS.KITV...........LY.VEGIIPE.P.KL..FLYQT..RIT.DK...VYNMVH.I...TFICH..T.. --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEYPPSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLSPSIIEKTMPSNLVNFILNAK ||||||| ||||||| | | |||||| ||||||| | |||||| ||||||||||| |||||||| |||||||| ||||||||||||||||||| FAVGSIS....IDLVYIK.Y.G.MNIISS.SVDFPEY...S.YIRGYN.PCGFVCSPMEE...YSKLVMFV.TEMRGKLS.SIIEKTMPSNLVNFILNAK -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 -------210-------220 DGIKAHRTPSRRGFHHNSHS |||||||| ||||| DGIKAHRT.SRRGF -------210----
Dihedral angle restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MDFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSKASRKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWDKSLQVYNMVHRIDSDTFICHTITQS ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| |||||||||||| ||||||||||| .DFKAIAQQTAQEVLGYNRDTSGWKVVKTSKKITVSSK..RKFHGNLYRVEGIIPESPAKLSDFLYQTGDRITWD.SLQVYNMVHRID.DTFICHTITQS --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEYPPSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTEMRGKLSPSIIEKTMPSNLVNFILNAK |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||| FAVGSISPRDFIDLVYIKRYEGNMNIISSKSVDFPEYPPSSNYIRGYNHPCGFVCSPMEENPAYSKLVMFVQTE....LSPSIIEKTMPSNLVNFILNAK -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 -------210-------220 DGIKAHRTPSRRGFHHNSHS ||||||||||||||| ||| DGIKAHRTPSRRGFH.NSH -------210---------