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BMRB: 19954

2MOW

Structure of Nrd1p CID - Trf4p NIM complex

Authors

Kabzinski, T., Stefl, R., Kubicek, K.

Assembly

Nrd1p CID - Trf4p NIM complex

Entity

1. Nrd1p CID - Trf4p NIM complex, entity 1 (polymer), 161 monomers, 18286.42 Da Detail

MQQDDDFQNF VATLESFKDL KSGISGSRIK KLTTYALDHI DIESKIISLI IDYSRLCPDS HKLGSLYIID SIGRAYLDET RSNSNSSSNK PGTCAHAINT LGEVIQELLS DAIAKSNQDH KEKIRMLLDI WDRSGLFQKS YLNAIRSKCF AMDLEHHHHH H

2. Nrd1p CID - Trf4p NIM complex, entity 2 (polymer, Thiol state: not present), 12 monomers, 1416.358 Da Detail

DDDEDGYNPY TL

Total weight

19702.777 Da

Max. entity weight

18286.42 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 68.1 %, Completeness (bb): 72.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.1 % (1367 of 2008)	76.0 % (792 of 1042)	54.0 % (422 of 781)	82.7 % (153 of 185)
Backbone	72.6 % (749 of 1032)	87.2 % (306 of 351)	57.7 % (295 of 511)	87.1 % (148 of 170)
Sidechain	64.5 % (736 of 1141)	70.3 % (486 of 691)	56.3 % (245 of 435)	33.3 % (5 of 15)
Aromatic	10.1 % (16 of 158)	20.3 % (16 of 79)	0.0 % (0 of 78)	0.0 % (0 of 1)
Methyl	86.1 % (155 of 180)	88.9 % (80 of 90)	83.3 % (75 of 90)

1. entity 1

MQQDDDFQNF VATLESFKDL KSGISGSRIK KLTTYALDHI DIESKIISLI IDYSRLCPDS HKLGSLYIID SIGRAYLDET RSNSNSSSNK PGTCAHAINT LGEVIQELLS DAIAKSNQDH KEKIRMLLDI WDRSGLFQKS YLNAIRSKCF AMDLEHHHHH H

2. entity 2

DDDEDGYNPY TL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 8.0

#	Name	Isotope labeling	Concentration
1	entity_2	natural abundance	1.5 mM
2	entity_1	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	100 mM
4	sodium phosphate	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹³C	water	methyl carbon	4.7 ppm	internal	direct	1.0
¹⁵N	water	nitrogen	4.7 ppm	internal	direct	1.0

Referencing offset: 2.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹³C	water	methyl carbon	4.7 ppm	internal	direct	1.0
¹⁵N	water	nitrogen	4.7 ppm	internal	direct	1.0

Referencing offset: 2.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹³C	water	methyl carbon	4.7 ppm	internal	direct	1.0
¹⁵N	water	nitrogen	4.7 ppm	internal	direct	1.0

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MOW, Strand ID: A, B Detail

Release date

2014-08-10

Citation

Molecular basis for coordinating transcription termination with noncoding RNA degradation
Tudek, A., Porrua, O., Kabzinski, T., Lidschreiber, M., Kubicek, K., Fortova, A., Lacroute, F., Vanacova, S., Cramer, P., Stefl, R., Libri, D.
Mol. Cell (2014), 55, 467-481, PubMed 25066235 , DOI 10.1016/j.molcel.2014.05.031 , Abstract

Related entities 1. Nrd1p CID - Trf4p NIM complex, entity 1, : 18 entities Detail

Related entities 2. Nrd1p CID - Trf4p NIM complex, entity 2, : 1 : 2 entities Detail

Interaction partners 2. Nrd1p CID - Trf4p NIM complex, entity 2, : 27 interactors Detail

More details for 27 interactors identified by 6 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC