BMRB: 20116

Substance P in DMPC:CHAPS q=0.25 bicelles

Authors

Gayen, A., Mukhopadhyay, C.

Assembly

Substance P in DMPC/CHAPS bicelles

Entity

1. Substance P in DMPC/CHAPS bicelles (polymer, Thiol state: not present), 11 monomers, 1348.612 Da Detail

RPKPQQFFGL M

Formula weight

1348.612 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts, conformer_family_coord_set

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 95.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.6 % (82 of 84)	97.6 % (82 of 84)
Backbone	95.2 % (20 of 21)	95.2 % (20 of 21)
Sidechain	98.4 % (62 of 63)	98.4 % (62 of 63)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. Substance P

RPKPQQFFGL M

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details substance p in DMPC/CHAPS q=0.25 bicelles pH 5.5 298 K

#	Name	Isotope labeling	Type	Concentration
1	Substance P	natural abundance		3.7 mM
2	DMPC/CHAPS bicelles	natural abundance		92.5 mM
3	H2O	natural abundance		0.0 ~ 0.0 mM

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Calculated from 5 models in PDB: 2KSA, Strand ID: B Detail

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Release date

2010-10-26

Citation

NMR evidence of GM1-induced conformational change of Substance P using isotropic bicelles
Gayen, A., Goswami, S., Mukhopadhyay, C.
Biochim. Biophys. Acta (2011), 1808, 127-139, PubMed 20937248 , DOI 10.1016/j.bbamem.2010.09.023 , Abstract

Related entities 1. Substance P in DMPC/CHAPS bicelles, : 1 : 20 : 1 entities Detail

Interaction partners 1. Substance P in DMPC/CHAPS bicelles, : 9 interactors Detail

Experiments performed 3 experiments Detail