BMRBj - BMREntryMaster

Found 1 Document (0.668 seconds)

BMRB: 20123

NS4A(1-22)

Authors

Montserret, R., Penin, F.

Assembly

NS4A(1-22)

Entity

1. NS4A(1-22) (polymer, Thiol state: all free), 22 monomers, 2139.471 Da Detail

STWVLVGGVL AALAAYCLST GS

Formula weight

2139.471 Da

Source organism

Hepacivirus C

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, conformer_family_coord_set

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.8 %, Completeness (bb): 76.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	82.8 % (164 of 198)	95.3 % (101 of 106)	68.5 % (63 of 92)
Backbone	76.4 % (84 of 110)	97.9 % (46 of 47)	60.3 % (38 of 63)
Sidechain	91.6 % (98 of 107)	93.2 % (55 of 59)	89.6 % (43 of 48)
Aromatic	94.7 % (18 of 19)	90.0 % (9 of 10)	100.0 % (9 of 9)
Methyl	95.0 % (38 of 40)	100.0 % (20 of 20)	90.0 % (18 of 20)

1. NS4A(1-22)

STWVLVGGVL AALAAYCLST GS

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 (±0.5) K, Details 50% trifluoroethanol D2OH 50% H2O (v/v)

#	Name	Isotope labeling	Concentration
1	NS4A(1-22)	natural abundance	0.9 (±0.05) mM
2	H2O	natural abundance	50 %
3	trifluoroethanol	natural abundance	50 %

Release date

2010-03-01

Citation

Structural determinants for membrane association and dynamic organization of the hepatitis C virus NS3-4A complex
Brass, V., Berke, J., Montserret, R., Blum, H., Penin, F., Moradpour, D.
Proc. Natl. Acad. Sci. U. S. A. (2008), 105, 14545-14550, PubMed 18799730 , DOI 10.1073/pnas.0807298105 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 15580 released on 2008-09-04
    Title NS4A(1-21)
  BMRB: 15582 released on 2008-09-04
    Title NS3(10-24)

Related entities 1. NS4A(1-22), : 2 : 7 : 8 : 9 entities Detail

Interaction partners 1. NS4A(1-22), : 5 interactors Detail

More details for 5 interactors identified by 4 citations

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr20123_3.str
Input source #2: Coordindates	bmr20123.coor.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): 1 vs Auth_asym_ID (model): A

------------10--------20--    
....STWVLVGGVLAALAAYCLSTGS    
    ||||||||||||||||||||||    
KKGGSTWVLVGGVLAALAAYCLSTGSGGKK
--------10--------20--------30

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
1	A	30	8	0	73.3

Content subtype: bmr20123_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	198		100.0 (chain: 1, length: 22)
1	Chemical shift references	chemical_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 22, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 140
  - ¹³C chemical shifts: 58
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	106	100	94.3
¹³C chemical shifts	92	58	63.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	46	97.9
¹³C chemical shifts	44	18	40.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	54	91.5
¹³C chemical shifts	48	40	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	20	20	100.0
¹³C chemical shifts	20	16	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	9	90.0
¹³C chemical shifts	9	9	100.0

Keywords membrane helix

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Found 1 Document (0.668 seconds)

BMRB: 20123

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. NS4A(1-22), : 2 : 7 : 8 : 9 entities Detail

Interaction partners 1. NS4A(1-22), : 5 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail