Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIR
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 11.2 % (74 of 662) | 11.2 % (74 of 662) |
Backbone | 18.5 % (42 of 227) | 18.5 % (42 of 227) |
Sidechain | 7.4 % (32 of 435) | 7.4 % (32 of 435) |
Aromatic | 15.7 % (11 of 70) | 15.7 % (11 of 70) |
Methyl | 3.6 % (2 of 55) | 3.6 % (2 of 55) |
1. barnase
AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIRTemperature 310 K, pH 4.5