Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIR
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 13.4 % (89 of 662) | 13.4 % (89 of 662) |
Backbone | 22.9 % (52 of 227) | 22.9 % (52 of 227) |
Sidechain | 8.5 % (37 of 435) | 8.5 % (37 of 435) |
Aromatic | 7.1 % (5 of 70) | 7.1 % (5 of 70) |
Methyl | 16.4 % (9 of 55) | 16.4 % (9 of 55) |
1. barnase
AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIRTemperature 300 K, pH 4.5