MDCSGCSRPG + Zn from basic conditions - tridentate binding
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | peptide | sing | 1:MET1:N | 1:GLY10:C |
2 | metal coordination | sing | 1:MET1:SD | 2:ZEM1:ZN |
3 | metal coordination | sing | 1:CYS6:SG | 2:ZEM1:ZN |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 95.9 % (47 of 49) | 95.9 % (47 of 49) |
Backbone | 100.0 % (21 of 21) | 100.0 % (21 of 21) |
Sidechain | 92.9 % (26 of 28) | 92.9 % (26 of 28) |
1. MDCSGCSRPG+Zn-basic
MDCSGCSRPGSolvent system 10% TDW/d6-DMSO, Temperature 298 (±0.1) K, Details The apo peptide (4.0 mg, 4.02 umol) was dissolved in TDW (1000 uL) and the pH was adjusted to 8.5 +/- 0.3 with a fresh solution of NaOH 0.1 M. The sample was lyophilized and the peptide (2.0 mg) was dissolved in a mixture of DMSO-d6 (450 uL) and TDW (50 uL) and was used as a control solution. In a separate flask, the lyophilized peptide (2.0 mg) was dissolved in DMSO-d6 (450 uL) and a stock solution of ZnCl2 (50 uL, 40.0 mM) in TDW was added, giving a dilution factor of 10 and a final concentration of 4.0 mM.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MDCSGCSRPG | natural abundance | protein | 4.0 mM |
2 | ZnCl2 | natural abundance | 4.0 mM | |
3 | DMSO | [U-2H] | 90 % | |
4 | H2O | natural abundance | 10 % |