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BMRB: 25033

2MQH

Solution structure of the Chlamydomonas reinhardtii NAB1 cold shock domain, CSD1

Authors

Sawyer, A., Mobli, M.

Assembly

Chlamydomonas reinhardtii NAB1 cold shock domain, CSD1

Entity

1. Chlamydomonas reinhardtii NAB1 cold shock domain, CSD1 (polymer, Thiol state: not present), 82 monomers, 8982.687 Da Detail

MRGSHHHHHH GSGEQLRQQG TVKWFNATKG FGFITPGGGG EDLFVHQTNI NSEGFRSLRE GEVVEFEVEA GPDGRSKAVN VT

Formula weight

8982.687 Da

Source organism

Chlamydomonas reinhardtii

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.8 %, Completeness: 77.2 %, Completeness (bb): 81.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.2 % (707 of 916)	77.7 % (369 of 475)	75.6 % (266 of 352)	80.9 % (72 of 89)
Backbone	81.4 % (397 of 488)	81.8 % (144 of 176)	80.6 % (187 of 232)	82.5 % (66 of 80)
Sidechain	73.8 % (366 of 496)	75.3 % (225 of 299)	71.8 % (135 of 188)	66.7 % (6 of 9)
Aromatic	34.0 % (34 of 100)	40.0 % (20 of 50)	26.5 % (13 of 49)	100.0 % (1 of 1)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. NAB1 cold shock domain

MRGSHHHHHH GSGEQLRQQG TVKWFNATKG FGFITPGGGG EDLFVHQTNI NSEGFRSLRE GEVVEFEVEA GPDGRSKAVN VT

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	NAB1 cold shock domain	[U-100% 13C; U-100% 15N]	267 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	300 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MQH, Strand ID: A Detail

Release date

2015-05-03

Citation 1

Solution structure of the RNA-binding cold-shock domain of the Chlamydomonas reinhardtii NAB1 protein and insights into RNA recognition
Sawyer, A., Mobli, M., Landsberg, M., Ross, I., Hankamer, B.
Biochem. J. (2015), 469, 97-106, PubMed 25919092 , DOI 10.1042/BJ20150217 , Abstract

Show more 1 citaion

Citation 2

NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii
Mussgnug, J., Wobbe, L., Elles, I., Claus, C., Hamilton, M., Fink, A., Kahmann, U., Kapazoglou, A., Mullineaux, C., Hippler, M., Nickelsen, J., Nixon, P., Kruse, O.
Plant Cell (2005), 17, 3409-3421, PubMed 16284312 , DOI 10.1105/tpc.105.035774 , Abstract

Hide non-primary 1 citaion