Structural Investigation of hnRNP L
DYGPHADSPV LMVYGLDQSK MNCDRVFNVF CLYGNVEKVK FMKSKPGAAM VEMADGYAVD RAITHLNNNF MFGQKMNVCV SKQPAIMPGQ SYGLEDGSCS YKDFSESRNN RFSTPEQAAK NRIQHPSNVL HFFNAPLEVT EENFFEICDE LGVKRPTSVK VFSGKSERSS SGLLEWDSKS DALETLGFLN HYQMKNPNGP YPYTLKLCFS TAQHAS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.8 % (1954 of 2513) | 83.9 % (1100 of 1311) | 65.8 % (640 of 973) | 93.4 % (214 of 229) |
Backbone | 80.4 % (1023 of 1272) | 93.5 % (406 of 434) | 65.3 % (414 of 634) | 99.5 % (203 of 204) |
Sidechain | 77.3 % (1115 of 1443) | 79.1 % (694 of 877) | 75.8 % (410 of 541) | 44.0 % (11 of 25) |
Aromatic | 48.8 % (121 of 248) | 62.9 % (78 of 124) | 34.1 % (42 of 123) | 100.0 % (1 of 1) |
Methyl | 97.7 % (172 of 176) | 96.6 % (85 of 88) | 98.9 % (87 of 88) |
1. entity
DYGPHADSPV LMVYGLDQSK MNCDRVFNVF CLYGNVEKVK FMKSKPGAAM VEMADGYAVD RAITHLNNNF MFGQKMNVCV SKQPAIMPGQ SYGLEDGSCS YKDFSESRNN RFSTPEQAAK NRIQHPSNVL HFFNAPLEVT EENFFEICDE LGVKRPTSVK VFSGKSERSS SGLLEWDSKS DALETLGFLN HYQMKNPNGP YPYTLKLCFS TAQHASSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 0.5 ~ 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | sodium phosphate | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 0.5 ~ 0.7 mM | |
8 | sodium chloride | natural abundance | 200 mM | |
9 | sodium phosphate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 0.5 ~ 0.7 mM | |
14 | sodium chloride | natural abundance | 200 mM | |
15 | sodium phosphate | natural abundance | 50 mM | |
16 | DTT | natural abundance | 1 mM | |
17 | D2O | natural abundance | 100 % |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Bruker Avance - 900 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 0.5 ~ 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | sodium phosphate | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Bruker Avance - 900 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 0.5 ~ 0.7 mM | |
8 | sodium chloride | natural abundance | 200 mM | |
9 | sodium phosphate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 900 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 0.5 ~ 0.7 mM | |
8 | sodium chloride | natural abundance | 200 mM | |
9 | sodium phosphate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Bruker Avance - 900 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 0.5 ~ 0.7 mM | |
14 | sodium chloride | natural abundance | 200 mM | |
15 | sodium phosphate | natural abundance | 50 mM | |
16 | DTT | natural abundance | 1 mM | |
17 | D2O | natural abundance | 100 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 0.5 ~ 0.7 mM | |
14 | sodium chloride | natural abundance | 200 mM | |
15 | sodium phosphate | natural abundance | 50 mM | |
16 | DTT | natural abundance | 1 mM | |
17 | D2O | natural abundance | 100 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_25040_2mqn.nef |
Input source #2: Coordindates | 2mqn.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
-------380-------390-------400-------410-------420-------430-------440-------450-------460-------470 DYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIMPGQSYGLEDGSCS |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| DYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIMPGQSYGLEDGSCS --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------480-------490-------500-------510-------520-------530-------540-------550-------560-------570 YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKSERSSSGLLEWDSKSDALETLGFLNHYQMKNPNGP |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKSERSSSGLLEWDSKSDALETLGFLNHYQMKNPNGP -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 -------580------ YPYTLKLCFSTAQHAS |||||||||||||||| YPYTLKLCFSTAQHAS -------210------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 216 | 0 | 0 | 100.0 |
Content subtype: combined_25040_2mqn.nef
Assigned chemical shifts
-------380-------390-------400-------410-------420-------430-------440-------450-------460-------470 DYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIMPGQSYGLEDGSCS ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| |||||||||||| DYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSK.GAAMVEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIM.GQSYGLEDGSCS -------480-------490-------500-------510-------520-------530-------540-------550-------560-------570 YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKSERSSSGLLEWDSKSDALETLGFLNHYQMKNPNGP |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||| YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKS.RSSSGLLEWDSKSDALETLGFLNHYQMKNPNGP -------580------ YPYTLKLCFSTAQHAS |||||||||||||||| YPYTLKLCFSTAQHAS
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1311 | 1081 | 82.5 |
13C chemical shifts | 973 | 587 | 60.3 |
15N chemical shifts | 236 | 216 | 91.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 434 | 402 | 92.6 |
13C chemical shifts | 432 | 186 | 43.1 |
15N chemical shifts | 204 | 203 | 99.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 877 | 679 | 77.4 |
13C chemical shifts | 541 | 401 | 74.1 |
15N chemical shifts | 32 | 13 | 40.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 97 | 95 | 97.9 |
13C chemical shifts | 97 | 94 | 96.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 124 | 78 | 62.9 |
13C chemical shifts | 123 | 42 | 34.1 |
15N chemical shifts | 1 | 1 | 100.0 |
Distance restraints
-------380-------390-------400-------410-------420-------430-------440-------450-------460-------470 DYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIMPGQSYGLEDGSCS ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| |||||||||| DYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSK.GAAMVEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIM...SYGLEDGSCS -------480-------490-------500-------510-------520-------530-------540-------550-------560-------570 YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKSERSSSGLLEWDSKSDALETLGFLNHYQMKNPNGP |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||| YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKS.RSSSGLLEWDSKSDALETLGFLNHYQMKNPNGP -------580------ YPYTLKLCFSTAQHAS |||||||||||||||| YPYTLKLCFSTAQHAS
Dihedral angle restraints
-------380-------390-------400-------410-------420-------430-------440-------450-------460-------470 DYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKMNVCVSKQPAIMPGQSYGLEDGSCS ||| ||||||| ||||| ||||||||||||||||| ||| |||||||||| ....................MNC.......CLYGNVE...FMKSK................AITHLNNNFMFGQKMNV.......IMP..SYGLEDGSCS -------480-------490-------500-------510-------520-------530-------540-------550-------560-------570 YKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPTSVKVFSGKSERSSSGLLEWDSKSDALETLGFLNHYQMKNPNGP | ||| ||||||||||||| ||| |||||| ||||||||| |||||| |||||| ||| Y.....SRN......EQAAKNRIQHPSN.......PLE..........DELGVK.......FSGKSERSS...LEWDSK..........NHYQMK.PNG. -------580------ YPYTLKLCFSTAQHAS |||||||||||||| ..YTLKLCFSTAQHAS