Structural Investigation of hnRNP L bound to RNA
GENYDDPHKT PASPVVHIRG LIDGVVEADL VEALQEFGPI SYVVVMPKKR QALVEFEDVL GACNAVNYAA DNQIYIAGHP AFVNYSTSQK ISRPGDSDDS RSVNS
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 79.1 % (995 of 1258) | 86.8 % (566 of 652) | 65.9 % (328 of 498) | 93.5 % (101 of 108) |
Backbone | 72.0 % (491 of 682) | 80.1 % (197 of 246) | 59.2 % (200 of 338) | 95.9 % (94 of 98) |
Sidechain | 89.2 % (601 of 674) | 90.9 % (369 of 406) | 87.2 % (225 of 258) | 70.0 % (7 of 10) |
Aromatic | 62.3 % (66 of 106) | 77.4 % (41 of 53) | 47.2 % (25 of 53) | |
Methyl | 100.0 % (120 of 120) | 100.0 % (60 of 60) | 100.0 % (60 of 60) |
1. entity 1
GENYDDPHKT PASPVVHIRG LIDGVVEADL VEALQEFGPI SYVVVMPKKR QALVEFEDVL GACNAVNYAA DNQIYIAGHP AFVNYSTSQK ISRPGDSDDS RSVNS2. RNA (5'-R(*CP*AP*CP*AP*CP*A)-3')
CACACASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-100% 15N] | 2 mM | |
2 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
3 | sodium chloride | natural abundance | 60 mM | |
4 | sodium phosphate | natural abundance | 40 mM | |
5 | DTT | natural abundance | 1 mM | |
6 | D2O | natural abundance | 10 % | |
7 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | entity_1 | [U-100% 13C; U-100% 15N] | 2 mM | |
9 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
10 | sodium chloride | natural abundance | 60 mM | |
11 | sodium phosphate | natural abundance | 40 mM | |
12 | DTT | natural abundance | 1 mM | |
13 | D2O | natural abundance | 10 % | |
14 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | entity_1 | [U-100% 15N] | 2 mM | |
16 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
17 | sodium chloride | natural abundance | 60 mM | |
18 | sodium phosphate | natural abundance | 40 mM | |
19 | DTT | natural abundance | 1 mM | |
20 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | entity_1 | [U-100% 13C; U-100% 15N] | 2 mM | |
22 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
23 | sodium chloride | natural abundance | 60 mM | |
24 | sodium phosphate | natural abundance | 40 mM | |
25 | DTT | natural abundance | 1 mM | |
26 | D2O | natural abundance | 100 % |
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 47.7 % (1199 of 2516) | 51.3 % (669 of 1304) | 41.6 % (414 of 996) | 53.7 % (116 of 216) |
Backbone | 46.3 % (631 of 1364) | 52.6 % (259 of 492) | 38.9 % (263 of 676) | 55.6 % (109 of 196) |
Sidechain | 50.2 % (677 of 1348) | 50.5 % (410 of 812) | 50.4 % (260 of 516) | 35.0 % (7 of 20) |
Aromatic | 42.5 % (90 of 212) | 50.0 % (53 of 106) | 34.9 % (37 of 106) | |
Methyl | 57.5 % (138 of 240) | 58.3 % (70 of 120) | 56.7 % (68 of 120) |
1. entity 1
GENYDDPHKT PASPVVHIRG LIDGVVEADL VEALQEFGPI SYVVVMPKKR QALVEFEDVL GACNAVNYAA DNQIYIAGHP AFVNYSTSQK ISRPGDSDDS RSVNS2. RNA (5'-R(*CP*AP*CP*AP*CP*A)-3')
CACACASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-100% 15N] | 2 mM | |
2 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
3 | sodium chloride | natural abundance | 60 mM | |
4 | sodium phosphate | natural abundance | 40 mM | |
5 | DTT | natural abundance | 1 mM | |
6 | D2O | natural abundance | 10 % | |
7 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | entity_1 | [U-100% 13C; U-100% 15N] | 2 mM | |
9 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
10 | sodium chloride | natural abundance | 60 mM | |
11 | sodium phosphate | natural abundance | 40 mM | |
12 | DTT | natural abundance | 1 mM | |
13 | D2O | natural abundance | 10 % | |
14 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | entity_1 | [U-100% 15N] | 2 mM | |
16 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
17 | sodium chloride | natural abundance | 60 mM | |
18 | sodium phosphate | natural abundance | 40 mM | |
19 | DTT | natural abundance | 1 mM | |
20 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310.15 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | entity_1 | [U-100% 13C; U-100% 15N] | 2 mM | |
22 | RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') | natural abundance | 2 mM | |
23 | sodium chloride | natural abundance | 60 mM | |
24 | sodium phosphate | natural abundance | 40 mM | |
25 | DTT | natural abundance | 1 mM | |
26 | D2O | natural abundance | 100 % |