NMR structure of UBA domain of DNA-damage-inducible 1 protein (Ddi1)
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 93.6 % (291 of 311) | 93.5 % (246 of 263) | 93.8 % (45 of 48) |
Backbone | 92.4 % (121 of 131) | 92.1 % (82 of 89) | 92.9 % (39 of 42) |
Sidechain | 94.4 % (170 of 180) | 94.3 % (164 of 174) | 100.0 % (6 of 6) |
Aromatic | 90.0 % (18 of 20) | 90.0 % (18 of 20) | |
Methyl | 96.0 % (24 of 25) | 96.0 % (24 of 25) |
1. entity
GSATFPEQTI KQLMDLGFPR DAVVKALKQT NGNAEFAASL LFQSSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298.2 K, pH 6.8, Details 1mM Ddi1UBA sample in pH6.8 20mM Sodium Phosphate Buffer, containing 7% D2O.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UBA | [U-100% 15N] | 1 mM | |
2 | D2O | [U-99% 2H] | 7 % | |
3 | H2O | natural abundance | 93 % | |
4 | sodium phosphate | natural abundance | 20 mM |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298.2 K, pH 6.8, Details 0.5mM Ddi1UBA sample in liquid crystalline medium
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | UBA | [U-100% 15N] | 0.5 mM | |
6 | D2O | [U-99% 2H] | 7 % | |
7 | H2O | natural abundance | 43 % | |
8 | sodium phosphate | natural abundance | 10 mM | |
9 | C12E5 | natural abundance | 5 % | |
10 | n-hexanol | natural abundance | 45 % |