Resonance assignments of myristoylated Y28F/Y67F mutant of the Mason-Pfizer monkey virus matrix protein
XGQELSQHER YVEQLKQALK TRGVKVKFAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYFNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.1 % (1402 of 1539) | 91.6 % (737 of 805) | 89.1 % (534 of 599) | 97.0 % (131 of 135) |
Backbone | 94.3 % (692 of 734) | 92.7 % (230 of 248) | 94.6 % (347 of 367) | 96.6 % (115 of 119) |
Sidechain | 89.1 % (823 of 924) | 91.0 % (507 of 557) | 85.5 % (300 of 351) | 100.0 % (16 of 16) |
Aromatic | 69.7 % (124 of 178) | 83.1 % (74 of 89) | 54.7 % (47 of 86) | 100.0 % (3 of 3) |
Methyl | 95.9 % (117 of 122) | 93.4 % (57 of 61) | 98.4 % (60 of 61) |
1. MPMV MA Y28F Y67F
XGQELSQHER YVEQLKQALK TRGVKVKFAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYFNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHHSolvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details the myristoyl residue is reverse-labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MPMV_MA_Y28F_Y67F | [U-99% 13C; U-99% 15N; NA-MYR] | 1.0 mM | |
2 | NaCl | natural abundance | 300.0 mM | |
3 | TCEP | natural abundance | 2.5 mM | |
4 | sodium phosphate | natural abundance | 50.0 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details the myristoyl residue and all histidine residues are reverse-labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | MPMV_MA_Y28F_Y67F | [U-99% 13C; U-99% 15N; NA-MYR,H] | 1.0 mM | |
8 | NaCl | natural abundance | 300.0 mM | |
9 | TCEP | natural abundance | 2.5 mM | |
10 | sodium phosphate | natural abundance | 50.0 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | MPMV_MA_Y28F_Y67F | [U-99% 13C; U-99% 15N] | 1.0 mM | |
14 | NaCl | natural abundance | 300.0 mM | |
15 | TCEP | natural abundance | 2.5 mM | |
16 | sodium phosphate | natural abundance | 50.0 mM | |
17 | H2O | natural abundance | 95 % | |
18 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details aminoacid residues are partially reverse-labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | MPMV_MA_Y28F_Y67F | [U-99% 13C]-MYR | 1.0 mM | |
20 | NaCl | natural abundance | 300.0 mM | |
21 | TCEP | natural abundance | 2.5 mM | |
22 | sodium phosphate | natural abundance | 50.0 mM | |
23 | H2O | natural abundance | 95 % | |
24 | D2O | natural abundance | 5 % |