BMRBj - BMREntryMaster

Found 1 Document (0.451 seconds)

BMRB: 25094

1MVF

full-length EcMazE-DNA complex

Authors

Zangger, K., van Nuland, N.A. J.

Assembly

EcMazE homodimer

Entity

1. EcMazE homodimer (polymer, Thiol state: not present), 82 monomers, 9355.612 × 2 Da Detail

MIHSSVKRWG NSPAVRIPAT LMQALNLNID DEVKIDLVDG KLIIEPVRKE PVFTLAELVN DITPENLHEN IDWGEPKDKE VW

Total weight

18711.225 Da

Max. entity weight

9355.612 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.8 %, Completeness: 26.3 %, Completeness (bb): 60.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	26.3 % (157 of 597)	15.9 % (81 of 511)	88.4 % (76 of 86)
Backbone	60.8 % (144 of 237)	44.7 % (72 of 161)	94.7 % (72 of 76)
Sidechain	3.6 % (13 of 360)	2.6 % (9 of 350)	40.0 % (4 of 10)
Aromatic	6.7 % (2 of 30)	3.7 % (1 of 27)	33.3 % (1 of 3)
Methyl	0.0 % (0 of 55)	0.0 % (0 of 55)

1. full EcMazE-DNA

MIHSSVKRWG NSPAVRIPAT LMQALNLNID DEVKIDLVDG KLIIEPVRKE PVFTLAELVN DITPENLHEN IDWGEPKDKE VW

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE-DNA	[U-99% 13C; U-99% 15N]	0.2 ~ 0.3 mM
2	DNA	natural abundance	0.0 ~ 0.4 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Release date

2015-02-01

Citation

Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
Zorzini, V., Buts, L., Schrank, E., Sterckx, Y.G. J., Respondek, M., Engelberg-Kulka, H., Loris, R., Zangger, K., van Nuland, N.A. J.
Nucleic Acids Res. (2015), 43, 1241-1256, PubMed 25564525 , DOI 10.1093/nar/gku1352 , Abstract

Related entities 1. EcMazE homodimer, : 1 : 3 : 5 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr25094_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr25094_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	159		87.8 (chain: 1, length: 82)
1	Chemical shift references	chemical_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 82, Sequence coverage: 87.8%
- Total number of assignments
  - ¹H chemical shifts: 81
  - ¹⁵N chemical shifts: 78
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	511	81	15.9
¹⁵N chemical shifts	89	78	87.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	161	72	44.7
¹⁵N chemical shifts	76	72	94.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	350	9	2.6
¹⁵N chemical shifts	13	6	46.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	57	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	1	3.7
¹⁵N chemical shifts	3	1	33.3

Keywords MazEF, intrinsic disorder, homodimer, Toxin-Antitoxin, transcription factor, DNA-binding

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Found 1 Document (0.451 seconds)

BMRB: 25094

Sample

Related entities 1. EcMazE homodimer, : 1 : 3 : 5 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail