Search

The NMR structure of the rubredoxin domain of the NO Reductase Flavorubredoxin from Escherichia coli
Authors
Turner, D.L., Silva, E., Lamosa, P.M., Teixeira, M.
Assembly
rubredoxin domain of the NO Reductase Flavorubredoxin
Entity
1. rubredoxin domain of the NO Reductase Flavorubredoxin (polymer, Thiol state: all other bound), 57 monomers, 6288.031 Da Detail

GPRMQCSVCQ WIYDPAKGEP MQDVAPGTPW SEVPDNFLCP ECSLGKDVFE ELASEAK


2. rubredoxin domain of the NO Reductase Flavorubredoxin, entity ZN (non-polymer), 65.409 Da
Total weight
6353.44 Da
Max. entity weight
6288.031 Da
Entity Connection
metal coordination 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1metal coordinationsing1:CYS6:SG2:ZN1:ZN
2metal coordinationsing1:CYS9:SG2:ZN1:ZN
3metal coordinationsing1:CYS39:SG2:ZN1:ZN
4metal coordinationsing1:CYS42:SG2:ZN1:ZN

Source organism
Escherichia coli
Exptl. method
solution NMR
Refine. method
torsion angle dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 94.7 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

Total1H15N
All94.7 % (373 of 394)94.4 % (319 of 338)96.4 % (54 of 56)
Backbone98.1 % (158 of 161)99.1 % (110 of 111)96.0 % (48 of 50)
Sidechain92.3 % (215 of 233)92.1 % (209 of 227)100.0 % (6 of 6)
Aromatic100.0 % (28 of 28)100.0 % (26 of 26)100.0 % (2 of 2)
Methyl100.0 % (21 of 21)100.0 % (21 of 21)

1. rubredoxin domain of the NO Reductase Flavorubredoxin

GPRMQCSVCQ WIYDPAKGEP MQDVAPGTPW SEVPDNFLCP ECSLGKDVFE ELASEAK

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.7


#NameIsotope labelingTypeConcentration
1rubredoxin domain of the NO Reductase Flavorubredoxin[U-99% 15N]1.1 mM
2sodium chloridenatural abundance100 mM
3potassium phosphatenatural abundance10 mM
4sodium azidenatural abundance0.04 %
5H2Onatural abundance90 %
6D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2MS3, Strand ID: A Detail


Release date
2015-07-19
Citation
Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase
Turner, D.L., Silva, E., Lamosa, P.M., Teixeira, M.
J. Mol. Biol. (2016), 428, 4686-4707, PubMed 27725182 , DOI 10.1016/j.jmb.2016.10.008 ,
Related entities 1. rubredoxin domain of the NO Reductase Flavorubredoxin, : 1 : 15 : 6 : 169 entities Detail
Interaction partners 1. rubredoxin domain of the NO Reductase Flavorubredoxin, : 7 interactors Detail
Experiments performed 5 experiments Detail
nullKeywords NorV, electron transport, flavorubredoxin, nitric oxide, nitric oxide reductase, rubredoxin