NMR structure of a putative phosphoglycolate phosphatase (NP_346487.1) from Streptococcus pneumoniae TIGR4
GHMQKTAFIW DLDGTLLDSY EAILSGIEET FAQFSIPYDK EKVREFIFKY SVQDLLVRVA EDRNLDVEVL NQVRAQSLAE KNAQVVLMPG AREVLAWADE SGIQQFIYTH KGNNAFTILK DLGVESYFTE ILTSQSGFVR KPSPEAATYL LDKYQLNSDN TYYIGDRTLD VEFAQNSGIQ SINFLESTYE GNHRIQALAD ISRIFETK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.4 % (2029 of 2461) | 85.2 % (1082 of 1270) | 75.3 % (724 of 962) | 97.4 % (223 of 229) |
Backbone | 71.7 % (889 of 1240) | 62.6 % (265 of 423) | 69.5 % (426 of 613) | 97.1 % (198 of 204) |
Sidechain | 94.2 % (1336 of 1418) | 96.5 % (817 of 847) | 90.5 % (494 of 546) | 100.0 % (25 of 25) |
Aromatic | 77.5 % (183 of 236) | 94.1 % (111 of 118) | 60.3 % (70 of 116) | 100.0 % (2 of 2) |
Methyl | 99.6 % (247 of 248) | 99.2 % (123 of 124) | 100.0 % (124 of 124) |
1. entity
GHMQKTAFIW DLDGTLLDSY EAILSGIEET FAQFSIPYDK EKVREFIFKY SVQDLLVRVA EDRNLDVEVL NQVRAQSLAE KNAQVVLMPG AREVLAWADE SGIQQFIYTH KGNNAFTILK DLGVESYFTE ILTSQSGFVR KPSPEAATYL LDKYQLNSDN TYYIGDRTLD VEFAQNSGIQ SINFLESTYE GNHRIQALAD ISRIFETKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 13C; U-100% 15N] | 1.5 (±0.1) mM | |
2 | D2O | [U-100% 2H] | 5 (±0.5) % | |
3 | sodium azide | natural abundance | 0.03 (±0.01) % | |
4 | sodium phosphate | natural abundance | 20 (±2.0) mM | |
5 | sodium chloride | natural abundance | 50 (±2.0) mM | |
6 | H2O | natural abundance | 95 % |