NMR structure of the III-IV-V three-way junction from the VS ribozyme
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.4 % (673 of 753) | 87.9 % (363 of 413) | 91.4 % (286 of 313) | 88.9 % (24 of 27) |
Suger, PO4 | 86.5 % (447 of 517) | 84.8 % (239 of 282) | 88.5 % (208 of 235) | |
Nucleobase | 95.8 % (226 of 236) | 94.7 % (124 of 131) | 100.0 % (78 of 78) | 88.9 % (24 of 27) |
Aromatic | 97.1 % (204 of 210) | 97.1 % (102 of 105) | 100.0 % (78 of 78) | 88.9 % (24 of 27) |
1. RNA (47-MER)
GGACCUCCCG UCCUUGGACG GUCGAGCGAA AGCUUGUGAU UGGUCCGSolvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | J345-1 | [U-100% 15N] | 1.5 ~ 2.0 mM | |
2 | KCl | natural abundance | 50 mM | |
3 | MgCl2 | natural abundance | 5 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | J345-2 | [U-100% 13C; U-100% 15N] | 2.0 mM | |
7 | KCl | natural abundance | 50 mM | |
8 | MgCl2 | natural abundance | 5 mM | |
9 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | J345-3 | [U-100% 13C; U-100% 15N] | 2.0 mM | |
11 | KCl | natural abundance | 50 mM | |
12 | MgCl2 | natural abundance | 5 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |