Three-Dimensional Structure and Interaction Studies of Hepatitis C Virus p7 in 1,2-Dihexanoyl-sn-glycero-3-phosphocholine by Solution Nuclear Magnetic Resonance
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 28.2 % (207 of 733) | 20.4 % (75 of 367) | 24.4 % (74 of 303) | 92.1 % (58 of 63) |
Backbone | 50.0 % (185 of 370) | 48.0 % (61 of 127) | 35.9 % (66 of 184) | 98.3 % (58 of 59) |
Sidechain | 6.2 % (26 of 421) | 5.8 % (14 of 240) | 6.8 % (12 of 177) | 0.0 % (0 of 4) |
Aromatic | 0.0 % (0 of 100) | 0.0 % (0 of 50) | 0.0 % (0 of 48) | 0.0 % (0 of 2) |
Methyl | 7.4 % (8 of 108) | 7.4 % (4 of 54) | 7.4 % (4 of 54) |
1. entity
ALENLVVLNA ASVAGAHGIL SFLVFFSAAW YIKGRLAPGA AYAFYGVWPL LLLLLALPPR AYASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 4.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | p7 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
2 | H2O | natural abundance | 90 % | |
3 | DHPC | natural abundance | 125 mM | |
4 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 4.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | p7 | [U-100% 15N] | 0.5 mM | |
6 | DHPC | natural abundance | 125 mM | |
7 | D2O | natural abundance | 10 % | |
8 | H2O | natural abundance | 90 % | |
9 | polyacrylamide gel | natural abundance | 6 % |