Solution Structure of the YTH Domain of YT521-B in complex with N6-Methyladenosine containing RNA
QTSKLKSVLQ DARFFLIKSN NHENVSLAKA KGVWSTLPVN EKKLNLAFRS ARSVILIFSV RESGKFQGFA RLSSESHHGG SPIHWVLPAG MSAKMLGGVF KIDWICRREL PFTKSAHLTN PWNEHKPVKI GRDGQEIELE CGTQLCLLFP PDESID
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.1 % (1732 of 1944) | 89.9 % (916 of 1019) | 87.1 % (661 of 759) | 93.4 % (155 of 166) |
Backbone | 88.6 % (864 of 975) | 86.4 % (298 of 345) | 88.0 % (424 of 482) | 95.9 % (142 of 148) |
Sidechain | 90.7 % (1010 of 1114) | 91.7 % (618 of 674) | 89.8 % (379 of 422) | 72.2 % (13 of 18) |
Aromatic | 69.6 % (128 of 184) | 77.2 % (71 of 92) | 61.6 % (53 of 86) | 66.7 % (4 of 6) |
Methyl | 98.8 % (166 of 168) | 100.0 % (84 of 84) | 97.6 % (82 of 84) |
1. entity 1
QTSKLKSVLQ DARFFLIKSN NHENVSLAKA KGVWSTLPVN EKKLNLAFRS ARSVILIFSV RESGKFQGFA RLSSESHHGG SPIHWVLPAG MSAKMLGGVF KIDWICRREL PFTKSAHLTN PWNEHKPVKI GRDGQEIELE CGTQLCLLFP PDESID2. RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3')
UGXCACSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 0.8 mM | |
2 | RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3') | natural abundance | 0.8 mM | |
3 | sodium phosphate | natural abundance | 25 mM | |
4 | sodium chloride | natural abundance | 25 mM | |
5 | beta-mercaptoethanol | natural abundance | 10 mM | |
6 | D2O | natural abundance | 10 % | |
7 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | entity_1 | [U-99% 13C; U-99% 15N] | 0.8 mM | |
9 | RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3') | natural abundance | 0.8 mM | |
10 | sodium phosphate | natural abundance | 25 mM | |
11 | sodium chloride | natural abundance | 25 mM | |
12 | beta-mercaptoethanol | natural abundance | 10 mM | |
13 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | entity_1 | [U-99% 15N] | 0.8 mM | |
15 | RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3') | natural abundance | 0.8 mM | |
16 | sodium phosphate | natural abundance | 25 mM | |
17 | sodium chloride | natural abundance | 25 mM | |
18 | beta-mercaptoethanol | natural abundance | 10 mM | |
19 | D2O | natural abundance | 100 % |
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 55.7 % (2164 of 3888) | 56.0 % (1141 of 2038) | 54.9 % (834 of 1518) | 56.9 % (189 of 332) |
Backbone | 56.8 % (1107 of 1950) | 56.7 % (391 of 690) | 56.0 % (540 of 964) | 59.5 % (176 of 296) |
Sidechain | 55.4 % (1234 of 2228) | 55.6 % (750 of 1348) | 55.8 % (471 of 844) | 36.1 % (13 of 36) |
Aromatic | 43.5 % (160 of 368) | 49.5 % (91 of 184) | 37.8 % (65 of 172) | 33.3 % (4 of 12) |
Methyl | 66.4 % (223 of 336) | 66.7 % (112 of 168) | 66.1 % (111 of 168) |
1. entity 1
QTSKLKSVLQ DARFFLIKSN NHENVSLAKA KGVWSTLPVN EKKLNLAFRS ARSVILIFSV RESGKFQGFA RLSSESHHGG SPIHWVLPAG MSAKMLGGVF KIDWICRREL PFTKSAHLTN PWNEHKPVKI GRDGQEIELE CGTQLCLLFP PDESID2. RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3')
UGXCACSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 0.8 mM | |
2 | RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3') | natural abundance | 0.8 mM | |
3 | sodium phosphate | natural abundance | 25 mM | |
4 | sodium chloride | natural abundance | 25 mM | |
5 | beta-mercaptoethanol | natural abundance | 10 mM | |
6 | D2O | natural abundance | 10 % | |
7 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | entity_1 | [U-99% 13C; U-99% 15N] | 0.8 mM | |
9 | RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3') | natural abundance | 0.8 mM | |
10 | sodium phosphate | natural abundance | 25 mM | |
11 | sodium chloride | natural abundance | 25 mM | |
12 | beta-mercaptoethanol | natural abundance | 10 mM | |
13 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | entity_1 | [U-99% 15N] | 0.8 mM | |
15 | RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3') | natural abundance | 0.8 mM | |
16 | sodium phosphate | natural abundance | 25 mM | |
17 | sodium chloride | natural abundance | 25 mM | |
18 | beta-mercaptoethanol | natural abundance | 10 mM | |
19 | D2O | natural abundance | 100 % |