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BMRB: 25221

2MUM

Solution structure of the PHD domain of Yeast YNG2

Authors

Taeb, S., Kaustov, L., Lemak, A., Farhadi, S., Sheng, Y.

Assembly

PHD domain of Yeast YNG2

Entity

1. PHD domain of Yeast YNG2, entity 1 (polymer), 50 monomers, 5932.778 Da Detail

TLYCFCQRVS FGEMVACDGP NCKYEWFHYD CVNLKEPPKG TWYCPECKIE

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

6063.5957 Da

Max. entity weight

5932.778 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.2 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.2 % (568 of 603)	97.1 % (306 of 315)	89.9 % (213 of 237)	96.1 % (49 of 51)
Backbone	95.2 % (278 of 292)	96.0 % (95 of 99)	94.6 % (139 of 147)	95.7 % (44 of 46)
Sidechain	94.1 % (337 of 358)	97.7 % (211 of 216)	88.3 % (121 of 137)	100.0 % (5 of 5)
Aromatic	82.2 % (74 of 90)	97.8 % (44 of 45)	65.1 % (28 of 43)	100.0 % (2 of 2)
Methyl	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)

1. entity 1

TLYCFCQRVS FGEMVACDGP NCKYEWFHYD CVNLKEPPKG TWYCPECKIE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	0.5 mM
2	sodium chloride	natural abundance	300 mM
3	potassium chloride	natural abundance	2.7 mM
4	potassium phosphate	natural abundance	2 mM
5	sodium phosphate	natural abundance	10 mM
6	CHAPS	natural abundance	0.05 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.35 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MUM, Strand ID: A Detail

Release date

2014-12-21

Citation

Solution structure of the PHD domain of Yeast YNG2
Taeb, S., Kaustov, L., Lemak, A., Farhadi, S., Sheng, Y.
Not known

Related entities 1. PHD domain of Yeast YNG2, entity 1, : 1 : 2 : 178 entities Detail

Interaction partners 1. PHD domain of Yeast YNG2, entity 1, : 32 interactors Detail

More details for 32 interactors identified by 9 citations

Experiments performed 10 experiments Detail

1 3D 1H-15N NOESY