BMRBj - BMREntryMaster

Found 1 Document (0.789 seconds)

BMRB: 25250

2MV8

Solution structure of Ovis Aries PrP with mutation delta190-197

Authors

Munoz, C., Egalon, A., Beringue, V., Rezaei, H., Dron, M., Sizun, C.

Assembly

PrPdelta190-197

Entity

1. PrPdelta190-197 (polymer, Thiol state: all disulfide bound), 146 monomers, 16644.40 Da Detail

MGSSHHHHHH SSGLVPRGSH MSNKPSKPKT NMKHVAGAAA AGAVVGGLGG YMLGSVMSRP LIHFGNDYED RYYRENMYRY PNQVYYRPVD QYSNQNNFVH DCVNITVKQG ENFTETDIKI MERVVEQMCI TQYQRESQAY YQRGAS

Formula weight

16644.4 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS102:SG	1:CYS129:SG

Source organism

Ovis aries

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 89.4 %, Completeness (bb): 91.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.4 % (1502 of 1681)	89.1 % (782 of 878)	89.4 % (576 of 644)	90.6 % (144 of 159)
Backbone	91.3 % (789 of 864)	90.6 % (271 of 299)	91.8 % (390 of 425)	91.4 % (128 of 140)
Sidechain	87.8 % (834 of 950)	88.3 % (511 of 579)	87.2 % (307 of 352)	84.2 % (16 of 19)
Aromatic	68.1 % (113 of 166)	71.1 % (59 of 83)	65.1 % (54 of 83)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. PrPdelta190-197

MGSSHHHHHH SSGLVPRGSH MSNKPSKPKT NMKHVAGAAA AGAVVGGLGG YMLGSVMSRP LIHFGNDYED RYYRENMYRY PNQVYYRPVD QYSNQNNFVH DCVNITVKQG ENFTETDIKI MERVVEQMCI TQYQRESQAY YQRGAS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 5.300

#	Name	Isotope labeling	Concentration
1	PrPdelta190-197	[U-99% 13C; U-99% 15N]	0.35 (±2.0E-5) mM
2	Sodium acetate	natural abundance	10.00 (±0.001) mM
3	H2O	natural abundance	90 (±0.001) mM
4	D2O	natural abundance	10 (±0.001) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 5.300

#	Name	Isotope labeling	Concentration
5	PrPdelta190-197	[U-99% 15N]	0.45 (±2.0E-5) mM
6	Sodium acetate	natural abundance	10.00 (±0.001) mM
7	H2O	natural abundance	90 (±0.001) mM
8	D2O	natural abundance	10 (±0.001) mM

Sample #3

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 5.300

#	Name	Isotope labeling	Concentration
9	PrPdelta190-197	[U-99% 13C; U-99% 15N]	0.35 (±0.02) mM
10	Sodium acetate	natural abundance	10.00 (±0.1) mM
11	D2O	natural abundance	100 (±0.001) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MV8, Strand ID: A Detail

Release date

2015-10-04

Citation

Conversion ability of PrP helix 2 deletion mutants
Munoz, C., Sizun, C., Egalon, A., Beringue, V., Rezaei, H., Dron, M.
Not known

Related entities 1. PrPdelta190-197, : 1 : 106 entities Detail

Interaction partners 1. PrPdelta190-197, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC