BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	74.5 % (251 of 337)	78.1 % (139 of 178)	66.4 % (89 of 134)	92.0 % (23 of 25)
Backbone	77.9 % (120 of 154)	88.9 % (48 of 54)	65.3 % (49 of 75)	92.0 % (23 of 25)
Sidechain	74.8 % (154 of 206)	73.4 % (91 of 124)	76.8 % (63 of 82)
Aromatic	26.3 % (10 of 38)	52.6 % (10 of 19)	0.0 % (0 of 19)
Methyl	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)

1. Molecule 1

MKFYTIKLPK FLGGIVRAML GSFRKD

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details 13C, 15N- SpoVM in 16 mM phopsphate (pH 6.), 80 mM NaCl, 16% deuterated DMPC/DHPC (q=0.3)

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2MVH, Strand ID: A Detail

Release date

2015-03-29

Citation

Structural and mechanistic basis for the geometric-driven subcellular localication of a small protein
Tian, F., Rill, R.L., Castaining, J., Hsin, J., Tan, I.S., Wang, X., Huang, K., Ramamurthi, K.S.
Nat. Struct. Biol.

Related entities 1. SpoVM, : 1 : 1 : 1 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceII - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details 13C, 15N- SpoVM in 16 mM phopsphate (pH 6.), 80 mM NaCl, 16% deuterated DMPC/DHPC (q=0.3)

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

2 2D 1H-13C HSQC

Instrument

Bruker AvanceII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

3 3D HNCO

Instrument

Bruker AvanceII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

4 3D HNCA

Instrument

Bruker AvanceII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

5 3D HNCACB

Instrument

Bruker AvanceII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

6 3D HCCH-TOCSY

Instrument

Bruker AvanceII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

7 3D 1H-15N NOESY

Instrument

Bruker AvanceII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

8 3D 1H-13C NOESY

Instrument

Bruker AvanceII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SpoVM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	16 mM
3	sodium chloride	natural abundance	80 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25268_2mvh.nef
Input source #2: Coordindates	2mvh.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20------
MKFYTIKLPKFLGGIVRAMLGSFRKD
||||||||||||||||||||||||||
MKFYTIKLPKFLGGIVRAMLGSFRKD

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	26	0	0	100.0

Content subtype: combined_25268_2mvh.nef

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list	Warning	251		100.0 (chain: A, length: 26)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_2	OK	20 (20)	.	46.2 (chain: A, length: 26)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 26, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 140
  - ¹³C chemical shifts: 89
  - ¹⁵N chemical shifts: 22
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	178	140	78.7
¹³C chemical shifts	134	89	66.4
¹⁵N chemical shifts	27	22	81.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	54	49	90.7
¹³C chemical shifts	52	26	50.0
¹⁵N chemical shifts	25	22	88.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	124	91	73.4
¹³C chemical shifts	82	63	76.8
¹⁵N chemical shifts	2	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	16	16	100.0
¹³C chemical shifts	16	16	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	10	52.6
¹³C chemical shifts	19	0	0.0

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 26, Sequence coverage: 46.2%
- Total number of restraints: 20
- Total number of restraints per polymer type: 20
- Weight of restraints: 1.0 (20)
- Potential type of restraints: square-well-parabolic (20)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue

Keywords SpoVM

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Found 1 Document (0.352 seconds)

BMRB: 25268

Sample

Related entities 1. SpoVM, : 1 : 1 : 1 entities Detail

Experiments performed 8 experiments Detail

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NMR combined restraints 4 contents Detail