BMRBj - BMREntryMaster

Found 1 Document (0.796 seconds)

BMRB: 25277

2MVO

Solution structure of the lantibiotic self-resistance lipoprotein MlbQ from Microbispora ATCC PTA-5024

Authors

Pozzi, R., Schwartz, P., Linke, D., Kulik, A., Nega, M., Wohlleben, W., Stegmann, E., Coles, M.

Assembly

lantibiotic self-resistance lipoprotein MlbQ from Microbispora ATCC PTA-5024

Entity

1. lantibiotic self-resistance lipoprotein MlbQ from Microbispora ATCC PTA-5024 (polymer), 147 monomers, 15432.89 Da Detail

HHHHHHSSGL VPRGSGMKET AAAKFERQHM DSPDLGTDDD DKTGGGRADP AHRSPVPLPS PTSNKQDISE ANLAYLWPLT VDHGTIECLP SDNAVFVAPD GTTYALNDRA EKAGHPPITP IRAKGSGGGY ISLGALLSTT LNLCGKG

Formula weight

15432.89 Da

Source organism

Microbispora sp. ATCC PTA-5024

Exptl. method

solution NMR

Refine. method

simulated annealing, NOESY back-calculation

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 76.2 %, Completeness: 68.0 %, Completeness (bb): 68.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.0 % (1066 of 1567)	67.2 % (544 of 809)	68.5 % (422 of 616)	70.4 % (100 of 142)
Backbone	68.5 % (586 of 856)	66.4 % (198 of 298)	69.6 % (295 of 424)	69.4 % (93 of 134)
Sidechain	68.8 % (579 of 841)	67.7 % (346 of 511)	70.2 % (226 of 322)	87.5 % (7 of 8)
Aromatic	44.8 % (43 of 96)	66.7 % (32 of 48)	21.3 % (10 of 47)	100.0 % (1 of 1)
Methyl	84.5 % (120 of 142)	84.5 % (60 of 71)	84.5 % (60 of 71)

1. MlbQ

HHHHHHSSGL VPRGSGMKET AAAKFERQHM DSPDLGTDDD DKTGGGRADP AHRSPVPLPS PTSNKQDISE ANLAYLWPLT VDHGTIECLP SDNAVFVAPD GTTYALNDRA EKAGHPPITP IRAKGSGGGY ISLGALLSTT LNLCGKG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0

#	Name	Isotope labeling	Concentration
1	MlbQ	[U-100% 15N]	0.5 mM
2	TRIS	natural abundance	20 mM
3	sodium chloride	natural abundance	250 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0

#	Name	Isotope labeling	Concentration
6	MlbQ	[U-100% 13C; U-100% 15N]	0.5 mM
7	TRIS	natural abundance	20 mM
8	sodium chloride	natural abundance	250 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MVO, Strand ID: A Detail

Release date

2015-07-12

Citation

Distinct mechanisms contribute to immunity in the lantibiotic NAI-107 producer strain Microbispora ATCC PTA-5024
Pozzi, R., Coles, M., Schwartz, P., Linke, D., Kulik, A., Nega, M., Wohlleben, W., Stegmann, E.
Environ. Microbiol. (2015), 18, 118-132, PubMed 25923468 , DOI 10.1111/1462-2920.12892 , Abstract

Related entities 1. lantibiotic self-resistance lipoprotein MlbQ from Microbispora ATCC PTA-5024, : 1 entities Detail

Experiments performed 13 experiments Detail

1 3D HNCO