Solution structure of 53BP1 tandem Tudor domains in complex with a p53K382me2 peptide
GHMNSFVGLR VVAKWSSNGY FYSGKITRDV GAGKYKLLFD DGYECDVLGK DILLCDPIPL DTEVTALSED EYFSAGVVKG HRKESGELYY SIEKEGQRKW YKRMAVILSL EQGNRLREQY GLG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.1 % (1492 of 1585) | 95.9 % (792 of 826) | 94.0 % (582 of 619) | 84.3 % (118 of 140) |
Backbone | 91.3 % (730 of 800) | 92.2 % (259 of 281) | 92.8 % (359 of 387) | 84.8 % (112 of 132) |
Sidechain | 97.5 % (881 of 904) | 97.8 % (533 of 545) | 97.4 % (342 of 351) | 75.0 % (6 of 8) |
Aromatic | 89.2 % (141 of 158) | 92.4 % (73 of 79) | 88.3 % (68 of 77) | 0.0 % (0 of 2) |
Methyl | 100.0 % (132 of 132) | 100.0 % (66 of 66) | 100.0 % (66 of 66) |
1. entity 1
GHMNSFVGLR VVAKWSSNGY FYSGKITRDV GAGKYKLLFD DGYECDVLGK DILLCDPIPL DTEVTALSED EYFSAGVVKG HRKESGELYY SIEKEGQRKW YKRMAVILSL EQGNRLREQY GLG2. entity 2
STSRHKXLMF KTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 25 mM | |
2 | sodium azide | natural abundance | 1.5 mM | |
3 | 53BP1-Tudor | [U-100% 13C; U-100% 15N] | 2.0 mM | |
4 | p53K382me2 | natural abundance | 6.0 mM | |
5 | D2O | natural abundance | 10 % | |
6 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | sodium phosphate | natural abundance | 25 mM | |
8 | sodium azide | natural abundance | 1.5 mM | |
9 | 53BP1-Tudor | [U-100% 13C; U-100% 15N] | 2.0 mM | |
10 | p53K382me2 | natural abundance | 6.0 mM | |
11 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | sodium phosphate | natural abundance | 25 mM | |
13 | sodium azide | natural abundance | 1.5 mM | |
14 | 53BP1-Tudor | natural abundance | 2.0 mM | |
15 | p53Kc382me2 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
16 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | sodium phosphate | natural abundance | 25 mM | |
18 | sodium azide | natural abundance | 1.5 mM | |
19 | 53BP1-Tudor | [U-100% 15N] | 2.0 mM | |
20 | p53K382me2 | natural abundance | 6.0 mM | |
21 | D2O | natural abundance | 10 % | |
22 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
23 | sodium phosphate | natural abundance | 25 mM | |
24 | sodium azide | natural abundance | 1.5 mM | |
25 | p53K382me2 | natural abundance | 2.0 mM | |
26 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
27 | sodium phosphate | natural abundance | 25 mM | |
28 | sodium azide | natural abundance | 1.5 mM | |
29 | p53Kc382me2 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
30 | D2O | natural abundance | 100 % |