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BMRB: 25358

Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum

Authors

Ding, X., Chen, C., Cui, Q., Li, W., Feng, Y.

Assembly

periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor

Entity

1. periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor (polymer, Thiol state: not present), 169 monomers, 18721.93 Da Detail

MYAYIDVDIN PSIGLVIDKK EKVIDAKPLN NDAKPILDEA APKDMPLYDA LSKILDISKK NGYINSADNI VLFSASINSG RNNVSESDKG IQEIISTLKD VAKDAGVKFE IIPSTEEDRQ KALDQNLSMG RYAIYVKAVE EGVNLNLEDA RNLSVSEILG KLEHHHHHH

Formula weight

18721.93 Da

Source organism

Acetivibrio thermocellus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.7 % (1907 of 1951)	97.5 % (990 of 1015)	97.9 % (741 of 757)	98.3 % (176 of 179)
Backbone	98.8 % (990 of 1002)	98.5 % (335 of 340)	99.2 % (495 of 499)	98.2 % (160 of 163)
Sidechain	96.7 % (1073 of 1110)	96.9 % (654 of 675)	96.4 % (404 of 419)	93.8 % (15 of 16)
Aromatic	76.1 % (70 of 92)	76.1 % (35 of 46)	76.1 % (35 of 46)
Methyl	99.0 % (206 of 208)	99.0 % (103 of 104)	99.0 % (103 of 104)

1. RsgI2-PD

MYAYIDVDIN PSIGLVIDKK EKVIDAKPLN NDAKPILDEA APKDMPLYDA LSKILDISKK NGYINSADNI VLFSASINSG RNNVSESDKG IQEIISTLKD VAKDAGVKFE IIPSTEEDRQ KALDQNLSMG RYAIYVKAVE EGVNLNLEDA RNLSVSEILG KLEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RsgI2-PD	[U-13C; U-15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	DSS	natural abundance	0.02 % w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 2 Detail

Release date

2015-06-03

Citation

Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum
Ding, X., Chen, C., Cui, Q., Li, W., Feng, Y.
Biomol. NMR Assign. (2015), 9, 321-324, PubMed 25682099 , DOI 10.1007/s12104-015-9601-7 , Abstract

Related entities 1. periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor, : 2 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC