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BMRB: 25391

6J2Y

1H, 15N and 13C resonance assignments of translationally-controlled tumor protein from Nannochloropsis oceanica

Authors

Yao, X., Feng, Y.

Assembly

tumor protein monomer

Entity

1. tumor protein monomer (polymer, Thiol state: all free), 188 monomers, 20591.91 Da Detail

MIVYKDVISG DEVVSDALKI TPVMEGGEEV PGLFEVDSAM VAVGGGDIDI GCGNAFGGAG DDEGADDATQ KENNVSGPSS FAYTAMPFSS KGEFKSWVKD YVRNVRQALK GSGVAVEDIK KFMEEAPTFV KWLVDKYDDL EFFMSKSMNP DAGLIFSYYK EGAHCPTFVY VKSGYKVVKF LEHHHHHH

Formula weight

20591.91 Da

Source organism

Nannochloropsis oceanica

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.6 %, Completeness: 85.2 %, Completeness (bb): 90.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.2 % (1813 of 2128)	84.6 % (925 of 1093)	85.1 % (719 of 845)	88.9 % (169 of 190)
Backbone	90.3 % (1006 of 1114)	88.7 % (345 of 389)	92.1 % (501 of 544)	88.4 % (160 of 181)
Sidechain	81.7 % (966 of 1182)	82.4 % (580 of 704)	80.4 % (377 of 469)	100.0 % (9 of 9)
Aromatic	46.6 % (110 of 236)	46.6 % (55 of 118)	45.7 % (53 of 116)	100.0 % (2 of 2)
Methyl	96.1 % (171 of 178)	94.4 % (84 of 89)	97.8 % (87 of 89)

1. NoTCTP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	NoTCTP	[U-13C; U-15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	5 mM
5	EDTA	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 % w/v
7	DSS	natural abundance	0.01 % w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 2 Detail

Release date

2015-06-03

Citation

(1)H, (15)N and (13)C resonance assignments of translationally-controlled tumor protein from photosynthetic microalga Nannochloropsis oceanica
Yao, X., Xiao, Y., Cui, Q., Feng, Y.
Biomol. NMR Assign. (2015), 9, 325-328, PubMed 25680850 , DOI 10.1007/s12104-015-9602-6 , Abstract

Related entities 1. tumor protein monomer, : 1 : 110 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC