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BMRB: 25405

2MXE

Solution structure of the C-terminal domain of MvaT

Authors

Ding, P., Xia, B.

Assembly

C-terminal domain of MvaT

Entity

1. C-terminal domain of MvaT (polymer, Thiol state: not present), 55 monomers, 6441.259 Da Detail

MKRARKVKQY KNPHTGEVIE TKGGNHKTLK EWKAKWGPEA VESWATLLGH HHHHH

Formula weight

6441.259 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.3 %, Completeness: 83.2 %, Completeness (bb): 85.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.2 % (561 of 674)	83.7 % (297 of 355)	81.9 % (213 of 260)	86.4 % (51 of 59)
Backbone	85.6 % (279 of 326)	86.7 % (98 of 113)	84.4 % (135 of 160)	86.8 % (46 of 53)
Sidechain	81.7 % (325 of 398)	82.2 % (199 of 242)	80.7 % (121 of 150)	83.3 % (5 of 6)
Aromatic	52.6 % (40 of 76)	52.6 % (20 of 38)	48.6 % (17 of 35)	100.0 % (3 of 3)
Methyl	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)

1. MvaT

MKRARKVKQY KNPHTGEVIE TKGGNHKTLK EWKAKWGPEA VESWATLLGH HHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.48 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MXE, Strand ID: A Detail

Release date

2015-06-28

Citation

A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT
Ding, P., McFarland, K.A., Jin, S., Tong, G., Duan, B., Yang, A., Hughes, T.R., Liu, J., Dove, S.L., Navarre, W.W., Xia, B.
PLoS Pathog. (2015), 11, e1004967-e1004967, PubMed 26068099 , DOI 10.1371/journal.ppat.1004967 , Abstract

Related entities 1. C-terminal domain of MvaT, : 2 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

4 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

5 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

6 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

7 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

8 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM

nullKeywords protein