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BMRB: 25407

2MXF

Structure of the DNA complex of the C-Terminal domain of MvaT

Authors

Ding, P., Xia, B.

Assembly

DNA complex of the C-Terminal domain of MvaT

Entity

1. MvaT (polymer, Thiol state: not present), 55 monomers, 6441.259 Da Detail

MKRARKVKQY KNPHTGEVIE TKGGNHKTLK EWKAKWGPEA VESWATLLGH HHHHH

2. DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') (polymer, Thiol state: not present), 12 monomers, 3725.376 × 2 Da Detail

CGCATATATG CG

Total weight

13892.011 Da

Max. entity weight

6441.259 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 76.4 %, Completeness (bb): 73.4 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	76.4 % (604 of 791)	81.6 % (385 of 472)	64.6 % (168 of 260)	86.4 % (51 of 59)
Backbone	73.4 % (301 of 410)	82.2 % (162 of 197)	58.1 % (93 of 160)	86.8 % (46 of 53)
Sidechain	80.3 % (346 of 431)	81.1 % (223 of 275)	78.7 % (118 of 150)	83.3 % (5 of 6)
Aromatic	64.0 % (64 of 100)	71.0 % (44 of 62)	48.6 % (17 of 35)	100.0 % (3 of 3)
Methyl	100.0 % (47 of 47)	100.0 % (25 of 25)	100.0 % (22 of 22)

1. MvaT

MKRARKVKQY KNPHTGEVIE TKGGNHKTLK EWKAKWGPEA VESWATLLGH HHHHH

2. DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')

CGCATATATG CG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	DNA (5'-D(CPGPCPAPTPAPTPAPTPGPCPG)-3')	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MXF, Strand ID: A Detail

Release date

2015-06-28

Citation

A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT
Ding, P., McFarland, K.A., Jin, S., Tong, G., Duan, B., Yang, A., Hughes, T.R., Liu, J., Dove, S.L., Navarre, W.W., Xia, B.
PLoS Pathog. (2015), 11, e1004967-e1004967, PubMed 26068099 , DOI 10.1371/journal.ppat.1004967 , Abstract

Related entities 1. MvaT, : 2 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC