BMRB: 25407

Structure of the DNA complex of the C-Terminal domain of MvaT

Authors

Ding, P., Xia, B.

Assembly

DNA complex of the C-Terminal domain of MvaT

Entity

1. MvaT (polymer, Thiol state: not present), 55 monomers, 6441.259 Da Detail

MKRARKVKQY KNPHTGEVIE TKGGNHKTLK EWKAKWGPEA VESWATLLGH HHHHH

2. DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') (polymer, Thiol state: not present), 12 monomers, 3725.376 × 2 Da Detail

CGCATATATG CG

Total weight

13892.011 Da

Max. entity weight

6441.259 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 76.4 %, Completeness (bb): 73.4 % Detail

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Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	1H	13C	15N
All	76.4 % (604 of 791)	81.6 % (385 of 472)	64.6 % (168 of 260)	86.4 % (51 of 59)
Backbone	73.4 % (301 of 410)	82.2 % (162 of 197)	58.1 % (93 of 160)	86.8 % (46 of 53)
Sidechain	80.3 % (346 of 431)	81.1 % (223 of 275)	78.7 % (118 of 150)	83.3 % (5 of 6)
Aromatic	64.0 % (64 of 100)	71.0 % (44 of 62)	48.6 % (17 of 35)	100.0 % (3 of 3)
Methyl	100.0 % (47 of 47)	100.0 % (25 of 25)	100.0 % (22 of 22)

1. MvaT

MKRARKVKQY KNPHTGEVIE TKGGNHKTLK EWKAKWGPEA VESWATLLGH HHHHH

2. DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')

CGCATATATG CG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	MvaT	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')	natural abundance		1 mM

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.101329

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Protein Blocks Logo

Calculated from 20 models in PDB: 2MXF, Strand ID: A Detail

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Release date

2015-06-28

Citation

A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT
Ding, P., McFarland, K.A., Jin, S., Tong, G., Duan, B., Yang, A., Hughes, T.R., Liu, J., Dove, S.L., Navarre, W.W., Xia, B.
PLoS Pathog. (2015), 11, e1004967-e1004967, PubMed 26068099 , DOI 10.1371/journal.ppat.1004967 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. MvaT, : 2 entities Detail

Experiments performed 7 experiments Detail