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BMRB: 25411

5LDL

MPMV MA T41I T78I

Authors

Kroupa, T., Hrabal, R.

Assembly

MPMV MA T41I T78I

Entity

1. MPMV MA T41I T78I (polymer, Thiol state: all free), 125 monomers, 14923.89 Da Detail

XGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD ICPWFPQEGT IDIKRWRRVG DCFQDYYNTF GPEKVPVIAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHH

Formula weight

14923.89 Da

Source organism

Mason-Pfizer monkey virus

Exptl. method

Refine. method

matrix relaxation, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 70.6 %, Completeness (bb): 79.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.6 % (1090 of 1545)	73.4 % (594 of 809)	65.1 % (391 of 601)	77.8 % (105 of 135)
Backbone	79.4 % (583 of 734)	89.9 % (223 of 248)	69.8 % (256 of 367)	87.4 % (104 of 119)
Sidechain	67.2 % (625 of 930)	66.1 % (371 of 561)	71.7 % (253 of 353)	6.3 % (1 of 16)
Aromatic	2.9 % (5 of 174)	5.7 % (5 of 87)	0.0 % (0 of 84)	0.0 % (0 of 3)
Methyl	93.7 % (118 of 126)	87.3 % (55 of 63)	100.0 % (63 of 63)

1. MPMV MA T41I T78I

XGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD ICPWFPQEGT IDIKRWRRVG DCFQDYYNTF GPEKVPVIAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

LACS Plot; CA

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 39 models in PDB: 2MXI, Strand ID: A Detail

Release date

2016-01-03

Citation

Mason-Pfizer monkey virus matrix protein mutants - structural view of impaired membrane interaction
Kroupa, T., Dolezal, M., Prchal, J., Ruml, T., Hrabal, R.
J. Mol. Biol.

Related entities 1. MPMV MA T41I T78I, : 1 : 2 : 23 entities Detail

Interaction partners 1. MPMV MA T41I T78I, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

4 3D HN(COCA)CB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

6 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details the myristoyl residue is reverse-labeled

#	Name	Isotope labeling	Type	Concentration
1	MPMV_MA_T41I_T78I	[U-99% 13C; U-99% 15N, NA-MYR]		0.4 ~ 0.7 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

nullKeywords M-PMV, Mason-Pfiser Monkey virus, matrix protein, myristoylation