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Structural features of a 3' splice site influenza A: 19-nt duplex
Authors
Turner, D.H., Kennedy, S.D., Chen, J.L.
Assembly
3' splice site influenza A: 19-nt duplex
Entity
1. 3' splice site influenza A: 19-nt duplex (polymer, Thiol state: not present), 19 monomers, 6234.724 Da Detail

GCAGGCCCAU GGGAGUGCA


Formula weight
6234.724 Da
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 83.6 %, Completeness (bb): 79.7 % Detail

Polymer type: polyribonucleotide

Total1H31P
All83.6 % (153 of 183)84.8 % (139 of 164)73.7 % (14 of 19)
Suger, PO479.7 % (106 of 133)80.7 % (92 of 114)73.7 % (14 of 19)
Nucleobase94.0 % (47 of 50)94.0 % (47 of 50)
Aromatic92.5 % (37 of 40)92.5 % (37 of 40)

1. RNA (19-MER)

GCAGGCCCAU GGGAGUGCA

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.0, Details 19-nt duplex


#NameIsotope labelingTypeConcentration
1entitynatural abundance4.0 mM
2H2Onatural abundance95 %
3D2Onatural abundance5 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.0, Details 19-nt duplex


#NameIsotope labelingTypeConcentration
4entitynatural abundance1.0 mM
5D2Onatural abundance100 %

Release date
2015-05-03
Citation
Structural features of a 3' splice site in influenza a
Chen, J.L., Kennedy, S.D., Turner, D.H.
Biochemistry (2015), 54, 3269-3285, PubMed 25909229 , DOI 10.1021/acs.biochem.5b00012 ,
Experiments performed 7 experiments Detail
nullKeywords influenza A, internal loop, segment 7, splice site