BMRB: 25416

Structural features of a 3' splice site in influenza A: 39-nt hairpin

Authors

Turner, D.H., Kennedy, S.D., Chen, J.L.

Assembly

RNA (39-MER), 3' splice site in influenza A: 39-nt hairpin

Entity

1. RNA (39-MER), 3' splice site in influenza A: 39-nt hairpin (polymer, Thiol state: not present), 39 monomers, 12751.59 Da Detail

GGAUUUGCAG GCCUACCAGA AACGGAUGGG AGUGCAGAU

Formula weight

12751.59 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 49.5 %, Completeness (bb): 33.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	49.5 % (165 of 333)	49.5 % (165 of 333)
Suger, PO4	33.3 % (78 of 234)	33.3 % (78 of 234)
Nucleobase	87.9 % (87 of 99)	87.9 % (87 of 99)
Aromatic	88.2 % (75 of 85)	88.2 % (75 of 85)

1. RNA (39-MER)

GGAUUUGCAG GCCUACCAGA AACGGAUGGG AGUGCAGAU

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.0, Details 39-nt hairpin

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		1.1 mM
2	H2O	natural abundance		95 %
3	D2O	natural abundance		5 %

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Release date

2015-05-03

Citation

Structural features of a 3' splice site in influenza a
Chen, J.L., Kennedy, S.D., Turner, D.H.
Biochemistry (2015), 54, 3269-3285, PubMed 25909229 , DOI 10.1021/acs.biochem.5b00012 , Abstract

Experiments performed 3 experiments Detail